Articles
EXPRESSION OF PATHOGENICITY-RELATED GENES OF APPLES IN RESPONSE TO ATTACK OF ERWINIA AMYLOVORA
Article number
1056_51
Pages
303 – 306
Language
English
Abstract
Pathogenesis-related (PR) genes play an important role in defense strategies of plants against biotic stresses.
In order to evaluate responses of apples to Erwinia amylovora, the expression of some candidate PR genes from the chitinase family including PR3-Ch2, PR3-Ch4, PR3-Ch5 and catalase-I was studied during 72 h of host/pathogen interaction.
In vitro grown shootlets of apple rootstocks MM-111 (resistant) and MM-106 (semi-susceptible) were inoculated with E. amylovora strain Ea273. RNA was extracted from samples collected at 0, 18, 31, 48 and 72 h after inoculation.
Specific primers for real-time expression studies were designed by using published sequences of candidate PR genes and elongation factor 1α (ef1α) was used as reference gene in all reactions.
The results showed that 31 h after inoculation a variation in expression of candidate defense genes in apples is detectable.
Interestingly, in MM-106, expression of catalase-I gene was significantly higher than that of more resistant apple, MM-111. Patterns of PR gene expressions were similar to the catalase-I gene and are likely subordinate to the generation of reactive oxygen by host cells.
In order to evaluate responses of apples to Erwinia amylovora, the expression of some candidate PR genes from the chitinase family including PR3-Ch2, PR3-Ch4, PR3-Ch5 and catalase-I was studied during 72 h of host/pathogen interaction.
In vitro grown shootlets of apple rootstocks MM-111 (resistant) and MM-106 (semi-susceptible) were inoculated with E. amylovora strain Ea273. RNA was extracted from samples collected at 0, 18, 31, 48 and 72 h after inoculation.
Specific primers for real-time expression studies were designed by using published sequences of candidate PR genes and elongation factor 1α (ef1α) was used as reference gene in all reactions.
The results showed that 31 h after inoculation a variation in expression of candidate defense genes in apples is detectable.
Interestingly, in MM-106, expression of catalase-I gene was significantly higher than that of more resistant apple, MM-111. Patterns of PR gene expressions were similar to the catalase-I gene and are likely subordinate to the generation of reactive oxygen by host cells.
Publication
Authors
M. Hassani, S.A. Salami, H. Abdollahi, L. Hamzaban
Keywords
pathogenesis related genes, host/pathogen interaction, chitinase-family genes
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