Articles
SOMACLONAL VARIATION: A USEFUL TOOL TO IMPROVE DISEASE RESISTANCE OF PEAR ROOTSTOCK ‘OLD HOME × FARMINGDALE’ (OHF 333) (PYRUS COMMUNIS L.)
Article number
1056_42
Pages
253 – 258
Language
English
Abstract
Somaclonal variation is a potentially useful source of genetic variability for plant improvement and could result in new valuable genotypes.
Several examples of somaclonal variation resulted in disease resistant forms have been reported in temperate fruit species.
In most of these cases, somaclonal variation was spontaneous and observed after regeneration from adventitious buds or somatic embryos.
The aim of our study was to obtain somaclones of pear (Pyrus communis L.) rootstock Old Home × Farmingdale (OHF 333) with resistance/tolerance to the fire blight.
The artificial inoculation of this rootstock with local strain of Erwinia amylovora showed that 50% of examined plants demonstrate low degree of visible symptoms of disease and 10% high degree of visible symptoms.
In order to obtain genotypes resistant/tolerant to E. amylovora we developed an efficient system for shoot regeneration from in vitro leaf explants.
All obtained regenerants were cloned, propagated, rooted and acclimatized to greenhouse conditions.
Somaclones were tested for resistance to fire blight (E. amylovora). It was found out that ten of the studied clones demonstrated a low susceptibility/tolerance to the fire blight.
The field tests of the selected genotypes are in progress.
Several examples of somaclonal variation resulted in disease resistant forms have been reported in temperate fruit species.
In most of these cases, somaclonal variation was spontaneous and observed after regeneration from adventitious buds or somatic embryos.
The aim of our study was to obtain somaclones of pear (Pyrus communis L.) rootstock Old Home × Farmingdale (OHF 333) with resistance/tolerance to the fire blight.
The artificial inoculation of this rootstock with local strain of Erwinia amylovora showed that 50% of examined plants demonstrate low degree of visible symptoms of disease and 10% high degree of visible symptoms.
In order to obtain genotypes resistant/tolerant to E. amylovora we developed an efficient system for shoot regeneration from in vitro leaf explants.
All obtained regenerants were cloned, propagated, rooted and acclimatized to greenhouse conditions.
Somaclones were tested for resistance to fire blight (E. amylovora). It was found out that ten of the studied clones demonstrated a low susceptibility/tolerance to the fire blight.
The field tests of the selected genotypes are in progress.
Publication
Authors
L.R. Nacheva, P.S. Gercheva, M.Y. Andonova, D.V. Panayotova , V.T. Dzhuvinov
Keywords
regeneration, fire blight, resistance, somaclones
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