Articles
PROTEOMIC ANALYSIS OF GRAFT COMPATIBILITY IN HETEROGRAFT COMBINATION OF CUCURBITACEAE PLANTS
Article number
1086_24
Pages
195 – 204
Language
English
Abstract
To understand the molecular mechanism takes place during heterograft combination of Cucurbitaceae plants, the seedlings of watermelon [Citrullus lanatus (Thunb.)] cultivar 8424 and melon (Cucumis melo L.) cultivar Yongtian No. 3 were grafted onto the compatible rootstock of winter squash (Cucurbita maxima duch. ex lam) A (TLZ-B2-1-24) to study the compatibility response of heterograft combination of watermelon/winter squash A (abb. W/A) and melon/winter squash A (abb. M/A), respectively.
The whole protein change during various grafting developmental stages was studied by SDS-PAGE electrophoresis.
A decrease in a 39 kDa callus protein band at the winter squash A rootstock was observed at 10 days after grafting, and the callus proteins of scion (watermelon and melon) were still kept.
Differentially expressed protein profile of the W(M)/A (No.5 day) and W(M)/A (No.20 day) were compared by 2-DE analysis.
The results showed there were 38 and 8 differentially expressed protein spots with a significant difference at P3.0 in W/A and M/A, respectively.
The number of protein spots belongs to the degradation, down-regulated, abundance and up-regulated was 27 (W/A+M/A, 23+4), 4 (W/A+M/A, 3+1), 5 (W/A+M/A, 2+3) and 10 (W/A+M/A, 10+0), respectively.
Sixteen degradation and down-regulated protein spots of W (M)/A (No.10 day) were analyzed by MALDI-TOF MS. Fourteen proteins were identified, which were involved in cellular process, metabolic process and signal transduction pathway.
Moreover, the fructokinase was detected in both W/A and M/A, which may indicated that sucrose metabolism played the important role during the compatible heterograft combination formation in Cucurbitaceae.
The whole protein change during various grafting developmental stages was studied by SDS-PAGE electrophoresis.
A decrease in a 39 kDa callus protein band at the winter squash A rootstock was observed at 10 days after grafting, and the callus proteins of scion (watermelon and melon) were still kept.
Differentially expressed protein profile of the W(M)/A (No.5 day) and W(M)/A (No.20 day) were compared by 2-DE analysis.
The results showed there were 38 and 8 differentially expressed protein spots with a significant difference at P3.0 in W/A and M/A, respectively.
The number of protein spots belongs to the degradation, down-regulated, abundance and up-regulated was 27 (W/A+M/A, 23+4), 4 (W/A+M/A, 3+1), 5 (W/A+M/A, 2+3) and 10 (W/A+M/A, 10+0), respectively.
Sixteen degradation and down-regulated protein spots of W (M)/A (No.10 day) were analyzed by MALDI-TOF MS. Fourteen proteins were identified, which were involved in cellular process, metabolic process and signal transduction pathway.
Moreover, the fructokinase was detected in both W/A and M/A, which may indicated that sucrose metabolism played the important role during the compatible heterograft combination formation in Cucurbitaceae.
Publication
Authors
H. Song, Y.H. Wang, X.Q. Zhang, Q.S. Ying, Y.E. Wang , L.Y. Yan
Keywords
winter squash, melon, watermelon, rootstock, heterograft, grafting protein
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