Articles
AN EFFICIENT IN VITRO MICROGRAFTING TECHNOLOGY OF WATERMELON
Article number
1086_6
Pages
65 – 70
Language
English
Abstract
In this study, we first developed an in vitro micro-grafting technology of watermelon [Citrullus lanatus (Thunb.) Matsum and Nakai] and in vitro culture factors were investigated to improve the survival rate.
Shoot tips excised from in vitro-raised seedlings were micro-grafted onto rootstocks.
We found that the survival rate was over 90% when scions (with length of 0.8 cm and 2 leaves) were grafted onto rootstocks (3 weeks old) using cleft grafting method, then the micrografted seedlings were cultivated on MS medium added with 5% sucrose for 20 days.
We hope that this study would lay a foundation for the theoretical research of watermelon grafting and cultivar improvement.
Shoot tips excised from in vitro-raised seedlings were micro-grafted onto rootstocks.
We found that the survival rate was over 90% when scions (with length of 0.8 cm and 2 leaves) were grafted onto rootstocks (3 weeks old) using cleft grafting method, then the micrografted seedlings were cultivated on MS medium added with 5% sucrose for 20 days.
We hope that this study would lay a foundation for the theoretical research of watermelon grafting and cultivar improvement.
Publication
Authors
N. Zhang, H.X. Zeng, X.F. Shi, Y.X. Yang, W.S. Cheng, J. Ren, Y.H. Li, A.C. Li, M. Tang, Y.H. Sun, D.X. Peng, L.Y. Chen
Keywords
watermelon, in vitro propagation, micrografting
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