Articles
ROLE OF PROTECTIVE ENZYMES IN TOMATO ROOTSTOCKS TO RESIST ROOT KNOT NEMATODES
Article number
1086_26
Pages
213 – 218
Language
English
Abstract
In recent years, root knot nematodes (RKN) are causing massive produce losses in vegetables.
Grafting can effectively reduce the root knot nematode disease if proper stocks resistant to this disease are used.
In this study, 11 tomato stocks from home and abroad were used to measure four kinds of protective enzymes including phenylalanine ammonia enzyme (PAL), polyphenol oxidase (PPO), peroxidase (POD) and superoxide dismutase (SOD), to differentiate the role of the protective enzymes play in the resistance to RKN. Results shows that LA0656 has the highest SOD level among all materials at 0.605 U/g FW, and broadly, the cultivars which have Mi gene have higher SOD activities and those without Mi gene have lower SOD activities. LA3601 have the highest levels of PPO activity, reaching 0.761 ΔOD/(g·min) FW, followed by CH101 and LA0656, while LA2822 and LA2826 nearly get a 0. LA2822 have highest PAL levels among all the cultivars, reaching 5.444 U/g FW, and there are some cultivars which get extremely low PAL levels, such as LA0655, LA0656, LA3858 and HA4402. POD enzyme levels of LA2822, LA3640 are almost 0, while the highest reached over 50ΔOD/(g·min) FW in LA0656 and LA2157. Our results indicate a complex relationship between enzyme activities and resistance, to help enclose what role the protective enzymes play in resistance mechanism of different tomato cultivars, choose better stocks and it also provide theoretical basis for further breeding work.
Grafting can effectively reduce the root knot nematode disease if proper stocks resistant to this disease are used.
In this study, 11 tomato stocks from home and abroad were used to measure four kinds of protective enzymes including phenylalanine ammonia enzyme (PAL), polyphenol oxidase (PPO), peroxidase (POD) and superoxide dismutase (SOD), to differentiate the role of the protective enzymes play in the resistance to RKN. Results shows that LA0656 has the highest SOD level among all materials at 0.605 U/g FW, and broadly, the cultivars which have Mi gene have higher SOD activities and those without Mi gene have lower SOD activities. LA3601 have the highest levels of PPO activity, reaching 0.761 ΔOD/(g·min) FW, followed by CH101 and LA0656, while LA2822 and LA2826 nearly get a 0. LA2822 have highest PAL levels among all the cultivars, reaching 5.444 U/g FW, and there are some cultivars which get extremely low PAL levels, such as LA0655, LA0656, LA3858 and HA4402. POD enzyme levels of LA2822, LA3640 are almost 0, while the highest reached over 50ΔOD/(g·min) FW in LA0656 and LA2157. Our results indicate a complex relationship between enzyme activities and resistance, to help enclose what role the protective enzymes play in resistance mechanism of different tomato cultivars, choose better stocks and it also provide theoretical basis for further breeding work.
Publication
Authors
L.K. Yin, W.C. Zhao, C. Shu, X.M. Li, J.W. Fan , S.H. Wang
Keywords
tomato, root knot nematode, enzyme activity, graft, rootstock
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