Articles
A LIQUID FORMULATION FOR BIOLOGICAL CONTROL OF ANTHRACNOSE IN RAMBUTAN
Article number
1091_14
Pages
117 – 123
Language
English
Abstract
The anthracnose pathogen Colletotrichum gloeosporioides, is a major cause of postharvest loss of rambutan in Sri Lanka.
Results of our investigation demonstrated antagonistic activity of Trichoderma asperellum against a rambutan isolate of C. gloeosporioides. The antagonistic activity was mainly due to coil formation around the pathogen hyphae by T. asperellum. Fungicidal activity was observed when rambutan fruit inoculated with 105 conidia/ml of C. gloeosporioides, were treated with the formulation containing spores of T. asperellum within 10 and 30 min after inoculation.
Treated fruit were observed to be free of disease when stored at 28±2°C for 7 days.
Fruit treated with the control formulation (formulation without conidia) and untreated control fruit showed characteristic symptoms of anthracnose disease.
Anthracnose symptoms were also observed on fruit that were inoculated with C. gloeosporioides and held as inoculated controls under similar storage conditions.
Chemical parameters including pH and titratable acidity among treated, untreated (controls) and fruit treated with the control formulation showed no significant differences.
The formulation was observed to be effective when stored at ambient temperature of 28±2°C for a period of up to 6 months.
The data suggest that a formulation of T. asperellum might be an alternate, eco-friendly method for the control of anthracnose disease of rambutan.
Results of our investigation demonstrated antagonistic activity of Trichoderma asperellum against a rambutan isolate of C. gloeosporioides. The antagonistic activity was mainly due to coil formation around the pathogen hyphae by T. asperellum. Fungicidal activity was observed when rambutan fruit inoculated with 105 conidia/ml of C. gloeosporioides, were treated with the formulation containing spores of T. asperellum within 10 and 30 min after inoculation.
Treated fruit were observed to be free of disease when stored at 28±2°C for 7 days.
Fruit treated with the control formulation (formulation without conidia) and untreated control fruit showed characteristic symptoms of anthracnose disease.
Anthracnose symptoms were also observed on fruit that were inoculated with C. gloeosporioides and held as inoculated controls under similar storage conditions.
Chemical parameters including pH and titratable acidity among treated, untreated (controls) and fruit treated with the control formulation showed no significant differences.
The formulation was observed to be effective when stored at ambient temperature of 28±2°C for a period of up to 6 months.
The data suggest that a formulation of T. asperellum might be an alternate, eco-friendly method for the control of anthracnose disease of rambutan.
Authors
C.J. Wijesinghe, R.S. Wilson Wijeratnam, R.L.C. Wijesundera, J.K.R.R. Samarasekara
Keywords
biocontrol, rambutan, Colletotrichum gloeosporioides, Trichoderma asperellum
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