Articles
AQUEOUS EXTRACTS FROM CALENDULA AS NATURAL POTENTIAL SOURCES TO DEVELOP PHYTO-MEDICINES AND NATURAL DEFENSE AGENTS
Article number
1098_12
Pages
117 – 123
Language
English
Abstract
Calendula officinalis L. (marigold) is a medicinal plant with healing, anti-microbial, and anti-inflammatory activities, especially for alcoholic extracts obtained from flowers, which contain mainly non-protein compounds as secondary metabolites.
Aqueous extracts of marigold are poorly studied.
Protein molecules, such as anti-microbial peptides (AMPs), could present activities of biotechnological interest.
AMPs are ubiquitous in nature and play an important role in plant defense, along with metabolites.
This study aimed to prospect an aqueous extract from dry flowers of marigold with potential antimicrobial activity for biotechnological use.
Native soluble extract (NSE) was obtained in aqueous solution, ultrafiltered using sequentially two membranes (10 and 1-kDa cut-off), and the low molecular weight fraction (NSE1-10kDa) was evaluated by antimicrobial activity assay and SDS-Tricine-PAGE. NSE1-10kDa was also precipitated with ammonium sulfate (between 35 and 75% saturation). The obtained fraction was separated by anionic exchange (AEC) and reversed phase (RPC) chromatography in HPLC, and the recovered peaks were analyzed by mass spectrometry (MS). The native fraction NSE1-10kDa inhibited the growth of Ralstonia solanacearum (gram-negative bacteria) and Clavibacter michiganensis subsp. michiganesis (gram-positive bacteria) in 22 and 26%, respectively, and peptide bands were observed on SDS-Tricine-PAGE. Cationic peaks recovered after AEC were separated by RPC, being eluted in the range from 32 to 48% (v/v) acetonitrile, similarly to other AMPs described.
MS analysis of NSE1-10kDa identified molecules with peptide masses, indicating that AMPs were present in the native sample and could account for the antimicrobial activity.
Aqueous extracts of marigold are poorly studied.
Protein molecules, such as anti-microbial peptides (AMPs), could present activities of biotechnological interest.
AMPs are ubiquitous in nature and play an important role in plant defense, along with metabolites.
This study aimed to prospect an aqueous extract from dry flowers of marigold with potential antimicrobial activity for biotechnological use.
Native soluble extract (NSE) was obtained in aqueous solution, ultrafiltered using sequentially two membranes (10 and 1-kDa cut-off), and the low molecular weight fraction (NSE1-10kDa) was evaluated by antimicrobial activity assay and SDS-Tricine-PAGE. NSE1-10kDa was also precipitated with ammonium sulfate (between 35 and 75% saturation). The obtained fraction was separated by anionic exchange (AEC) and reversed phase (RPC) chromatography in HPLC, and the recovered peaks were analyzed by mass spectrometry (MS). The native fraction NSE1-10kDa inhibited the growth of Ralstonia solanacearum (gram-negative bacteria) and Clavibacter michiganensis subsp. michiganesis (gram-positive bacteria) in 22 and 26%, respectively, and peptide bands were observed on SDS-Tricine-PAGE. Cationic peaks recovered after AEC were separated by RPC, being eluted in the range from 32 to 48% (v/v) acetonitrile, similarly to other AMPs described.
MS analysis of NSE1-10kDa identified molecules with peptide masses, indicating that AMPs were present in the native sample and could account for the antimicrobial activity.
Authors
L.C. Carrijo, A.E. Marques, P.P. Fontes, P.D. Games, M.J. Magalhães-Junior , M.C. Baracat-Pereira
Keywords
antimicrobial peptides, antimicrobial activity, flower extract, marigold, Calendula officinalis L.
Online Articles (17)