Articles
Micropropagation of Dendrobium cruentum Rchb. f., a rare Thai orchid species
Article number
1167_10
Pages
69 – 74
Language
English
Abstract
Shoot tips of Dendrobium cruentum Rchb. f. were cultured on 1/2 Murashige and Skoog (MS) liquid medium supplemented with 1% (w/v) sucrose and 1.0 ppm NAA. The survival rate, PLB (protocorm-like body) formation, number of PLBs shoot-1 and relative growth rate were 69, 69%, 1 PLB shoot-1 and 139%, respectively.
The highest production of shoots were observed on PLBs cultured on Vacin and Went (VW) agar medium supplemented with 1.5 ppm BA. This media gave the survival rate, shoot formation, number of shoots PLB-1, root formation and number of roots PLB-1 of 77, 75%, 34 shoots PLB-1, 63% and 27 roots PLB-1, respectively.
The highest production of shoots were observed on PLBs cultured on Vacin and Went (VW) agar medium supplemented with 1.5 ppm BA. This media gave the survival rate, shoot formation, number of shoots PLB-1, root formation and number of roots PLB-1 of 77, 75%, 34 shoots PLB-1, 63% and 27 roots PLB-1, respectively.
Authors
S. Sangdum, K. Thammasiri, N. Chuenboonngarm, K. Sopalun
Keywords
Dendrobium cruentum, 1/2 Murashige and Skoog (MS), Vacin and Went (VW), protocorm-like bodies, sucrose, NAA, BA
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