Articles
Asymbiotic seed germination and protocorm-like body proliferation of Cymbidium finlaysonianum Lindl.
Article number
1167_15
Pages
101 – 106
Language
English
Abstract
An in vitro plant regeneration protocol was successfully established for Cymbidium finlaysonianum Lindl., an epiphytic orchid by culturing seeds from six-month-old green self-pollinated fruits.
Tetrazolium (TZ) viability test indicated a mean embryo viability of 97.80%. Protocorm-like bodies (PLBs) of Cymbidium finlaysonianum Lindl. can be induced from protocorm segments cultured in VW liquid medium supplemented with 8.84 µM BAP and significantly different from other treatments, with the highest number of 5.2 PLBs explant-1. The PLBs converted into plants with well-developed shoots and roots on VW medium supplemented with 0.2% (w/v) activated charcoal (AC), without plant growth regulators after 2 months of culture.
The highest percentage of plantlet survival recorded at 2 months after hardening in the greenhouse was 85.8%.
Tetrazolium (TZ) viability test indicated a mean embryo viability of 97.80%. Protocorm-like bodies (PLBs) of Cymbidium finlaysonianum Lindl. can be induced from protocorm segments cultured in VW liquid medium supplemented with 8.84 µM BAP and significantly different from other treatments, with the highest number of 5.2 PLBs explant-1. The PLBs converted into plants with well-developed shoots and roots on VW medium supplemented with 0.2% (w/v) activated charcoal (AC), without plant growth regulators after 2 months of culture.
The highest percentage of plantlet survival recorded at 2 months after hardening in the greenhouse was 85.8%.
Authors
S. Rittirat, S. Klaocheed, S. Prasertsongskun, K. Thammasiri
Keywords
boat orchid, tetrazolium viability test, BAP, plantlets, hardening, activated charcoal
Online Articles (62)