Articles
In vitro microrhizome induction in Hedychium stenopetalum Lodd.
Article number
1167_25
Pages
163 – 168
Language
English
Abstract
Hedychium stenopetalum Lodd. is a potential species as a source for raw materials for pharmaceutical and cosmetic products.
In this study, the possibility of in vitro microrhizome induction in H. stenopetalum was studied.
Sterile young shoot tips were cultured on modified Murashige and Skoog medium (MS) (1962) supplemented with various concentrations of 6-benzylaminopurine (BAP) at 0, 1, 2, 3 and 4 mg L-1. Cultures were incubated under 16 h illumination day-1 at 25±2°C for 8 weeks.
The 1 mg L-1 BAP supplemented MS medium increased both number of shoots explant-1 and shoot height with higher levels showing no further increase in number of shoots and decreased shoot height.
Six-week-old in vitro plantlets were selected as materials for an experiment on microrhizome induction.
Plantlets were cultured on modified MS medium supplemented with sucrose concentrations of 3, 6, 9 and 12% in combination with ancymidol at 0, 1, 2, 3 and 4 μM. The heaviest microrhizome production was obtained from medium supplemented with 6 and 9% sucrose.
Ancymidol had no significant effect on microrhizome induction.
These results will be used to develop protocols for large-scale production of uniform and pathogen-free H. stenopetalum microrhizomes for field plantations, germplasm conservation and large-scale production of rhizomes for the herbal cosmetics and pharmaceutical industries.
In this study, the possibility of in vitro microrhizome induction in H. stenopetalum was studied.
Sterile young shoot tips were cultured on modified Murashige and Skoog medium (MS) (1962) supplemented with various concentrations of 6-benzylaminopurine (BAP) at 0, 1, 2, 3 and 4 mg L-1. Cultures were incubated under 16 h illumination day-1 at 25±2°C for 8 weeks.
The 1 mg L-1 BAP supplemented MS medium increased both number of shoots explant-1 and shoot height with higher levels showing no further increase in number of shoots and decreased shoot height.
Six-week-old in vitro plantlets were selected as materials for an experiment on microrhizome induction.
Plantlets were cultured on modified MS medium supplemented with sucrose concentrations of 3, 6, 9 and 12% in combination with ancymidol at 0, 1, 2, 3 and 4 μM. The heaviest microrhizome production was obtained from medium supplemented with 6 and 9% sucrose.
Ancymidol had no significant effect on microrhizome induction.
These results will be used to develop protocols for large-scale production of uniform and pathogen-free H. stenopetalum microrhizomes for field plantations, germplasm conservation and large-scale production of rhizomes for the herbal cosmetics and pharmaceutical industries.
Authors
S. Rodpradit, K. Songnun, K. Shusuwanaruk
Keywords
ginger lily, micropropagation, 6-benzylaminopurine, ancymidol, sucrose
Online Articles (62)