Articles
Role of plant growth regulators on fruit set and embryo culture of interspecific Phalaenopsis
Article number
1167_18
Pages
119 – 126
Language
English
Abstract
Phalaenopsis buyssoniana is a species in the Phalaenopsis genus that has high chromosome numbers (2n=4x=76); however, the problem occurred when crossed with P. ‘Wedding Promenade’. The cross has low fruit set.
In order to overcome this restriction, plant growth regulators were used in this study for 2 phases; 1) applying in vivo after cross pollination to promote fruit set; and 2) adding into the culture medium to enhance efficiency of embryo rescue.
Auxin treatments (NAA, IAA or 2,4-D) were applied after cross pollination to promote fruit set of P. buyssoniana and P. ‘Wedding Promenade’. P. ‘Wedding Promenade’, a female parent, produced the highest fruit set, increased capsule size and rescued embryos when 2,4-D was applied.
The study of capsule age and culture medium were conducted in order to enhance efficiency of embryo rescue.
The capsule ages were 1, 1.5, 2 and 2.5 months.
The culture media were based on Vacin and Went medium (control) and the other 4 treatments were 1) 100 mg L-1 2,4-D; 2) 50 mg L-1 Dicamba; 3) 1 mg L-1 Kinetin plus 0.1 mg L-1 NAA; and 4) 2 g L-1 Peptone.
The two-month-old capsules, cultured in Vacin and Went and supplemented with 1 mg L-1 Kinetin plus 0.1 mg L-1 of NAA, were found highly effective, producing 100% capsule development with 82.6 developed embryos per capsule.
In order to overcome this restriction, plant growth regulators were used in this study for 2 phases; 1) applying in vivo after cross pollination to promote fruit set; and 2) adding into the culture medium to enhance efficiency of embryo rescue.
Auxin treatments (NAA, IAA or 2,4-D) were applied after cross pollination to promote fruit set of P. buyssoniana and P. ‘Wedding Promenade’. P. ‘Wedding Promenade’, a female parent, produced the highest fruit set, increased capsule size and rescued embryos when 2,4-D was applied.
The study of capsule age and culture medium were conducted in order to enhance efficiency of embryo rescue.
The capsule ages were 1, 1.5, 2 and 2.5 months.
The culture media were based on Vacin and Went medium (control) and the other 4 treatments were 1) 100 mg L-1 2,4-D; 2) 50 mg L-1 Dicamba; 3) 1 mg L-1 Kinetin plus 0.1 mg L-1 NAA; and 4) 2 g L-1 Peptone.
The two-month-old capsules, cultured in Vacin and Went and supplemented with 1 mg L-1 Kinetin plus 0.1 mg L-1 of NAA, were found highly effective, producing 100% capsule development with 82.6 developed embryos per capsule.
Authors
K. Rungruchkanont, T. Promchot
Keywords
auxin, embryo rescue, fruit set, hybrid, Phalaenopsis orchid
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