Articles
Effect of in vitro thermotherapy application on shoot development in some fig cultivars
Article number
1173_33
Pages
195 – 198
Language
English
Abstract
In this study, at the end of the in vitro thermotherapy application in fig (Ficus carica L.), shoot viability ratio and status of shoot development were aimed to determine.
In the present research, shoot tips (0.7-1.0 cm) of ‘Sarılop’, ‘Bursa Siyahı’ female and ‘Ak ilek’, ‘Kaba ilek’ male fig cultivars at Fig Research Institute, Erbeyli, Aydın, were cultivated in MS (Murashige and Skoog, 1962) medium including 2.0 mg L-1 Benzyladenine (BA), 0.2 mg L-1 gibberellic acid (GA3), 0.1 mg L-1 indole butyric acid (IBA), and 89 mg L-1 phloroglucinol (PG). In the beginning of the thermotherapy application, relative humidity and temperature were 70% RH and 27°C, respectively, and the temperature was increased to 37°C in increasement of 2°C every day.
After that, temperature was kept constant.
After three-weeks of thermotherapy application, shoot tips taken from viable shoots were transferred to fresh medium.
After five weeks in culture, developed shoot numbers were determined.
In the present research, shoot tips (0.7-1.0 cm) of ‘Sarılop’, ‘Bursa Siyahı’ female and ‘Ak ilek’, ‘Kaba ilek’ male fig cultivars at Fig Research Institute, Erbeyli, Aydın, were cultivated in MS (Murashige and Skoog, 1962) medium including 2.0 mg L-1 Benzyladenine (BA), 0.2 mg L-1 gibberellic acid (GA3), 0.1 mg L-1 indole butyric acid (IBA), and 89 mg L-1 phloroglucinol (PG). In the beginning of the thermotherapy application, relative humidity and temperature were 70% RH and 27°C, respectively, and the temperature was increased to 37°C in increasement of 2°C every day.
After that, temperature was kept constant.
After three-weeks of thermotherapy application, shoot tips taken from viable shoots were transferred to fresh medium.
After five weeks in culture, developed shoot numbers were determined.
Publication
Authors
G. Günver Dalkılıç , B. Ertan, S. Dağ, P. Görücüoğlu
Keywords
fig, Ficus carica L., in vitro thermotherapy, shoot tip
Groups involved
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