Articles
Apocarotenoid gene expression in in vitro developed stigma-like structures in Crocus sativus L.
Article number
1200_8
Pages
51 – 54
Language
English
Abstract
Stigma-like structures (SLS) were produced under in vitro conditions.
Highest response was observed with half ovaries on G-5 media supplemented with different combinations of phytohormones.
The relative quantification through real time PCR for expression of apocarotenoid genes like CsBCH and CsGT-2 revealed that there is increase in expression from callus to SLS development.
Expression pattern of CsBCH and CsGT-2 studied in different flower parts revealed highest expression in stigma followed by SLS, style and tepal.
Expression of the regulatory genes responsible for biosynthesis of apocarotenoids viz; crocin, picrocrocin and safranal is getting upregulated in SLS in saffron hence revealing that these structures are developmentally closely related to natural stigma.
Highest response was observed with half ovaries on G-5 media supplemented with different combinations of phytohormones.
The relative quantification through real time PCR for expression of apocarotenoid genes like CsBCH and CsGT-2 revealed that there is increase in expression from callus to SLS development.
Expression pattern of CsBCH and CsGT-2 studied in different flower parts revealed highest expression in stigma followed by SLS, style and tepal.
Expression of the regulatory genes responsible for biosynthesis of apocarotenoids viz; crocin, picrocrocin and safranal is getting upregulated in SLS in saffron hence revealing that these structures are developmentally closely related to natural stigma.
Authors
J.I. Mir, N. Ahmed, R. Kumar, W. Shafi, S.H. Wani, R. Rashid, M.A. Sheikh
Keywords
saffron, SLS, Cs BCH, CsGT-2, real time PCR
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