Articles
Analysis of S-incompatibility in Campomanesia xanthocarpa (Myrtaceae)
Article number
1231_16
Pages
91 – 98
Language
English
Abstract
Campomanesia xanthocarpa (Myrtaceae), popularly known as guabirobeira, is a neglected native fruit species of the Brazilian Atlantic Forest Biome, a biodiversity hotspot.
In the present work we studied its reproductive biology associated with features of its pollination, and the occurrence of self-incompatibility.
The plant material originated from plants located in Alfredo Wagner City, Santa Catarina State, in southern Brazil.
Controlled pollination and the collection of buds were held to evaluate the reproductive system.
Observations of pollen tube growth at different collection times were carried out.
In controlled cross-pollination, germination began within two hours, and pollen tubes were observed growing in an organized pattern, from the stigma towards the ovary and then fertilizing 100% of the flowers.
This system showed no barriers 72 h after pollination.
In the self-pollination experiments, germination started 16 h later.
In contrast to cross-pollination, pollen tubes grew in a disorganized pattern into the style only in 40% of the flowers, and no penetration into the ovules was observed i.e., there was no fertilization.
These results demonstrate inhibition of self-pollen tube growth and suggest the occurrence of homomorphic gametophytic self-incompatibility (GSI). New studies are in progress to evaluate the molecular basis of S-incompatibility in this species.
In the present work we studied its reproductive biology associated with features of its pollination, and the occurrence of self-incompatibility.
The plant material originated from plants located in Alfredo Wagner City, Santa Catarina State, in southern Brazil.
Controlled pollination and the collection of buds were held to evaluate the reproductive system.
Observations of pollen tube growth at different collection times were carried out.
In controlled cross-pollination, germination began within two hours, and pollen tubes were observed growing in an organized pattern, from the stigma towards the ovary and then fertilizing 100% of the flowers.
This system showed no barriers 72 h after pollination.
In the self-pollination experiments, germination started 16 h later.
In contrast to cross-pollination, pollen tubes grew in a disorganized pattern into the style only in 40% of the flowers, and no penetration into the ovules was observed i.e., there was no fertilization.
These results demonstrate inhibition of self-pollen tube growth and suggest the occurrence of homomorphic gametophytic self-incompatibility (GSI). New studies are in progress to evaluate the molecular basis of S-incompatibility in this species.
Authors
C.M. Ribas-Santos, M. Santos, M.P. Guerra
Keywords
Atlantic forest biome, reproductive biology, underutilized fruit species
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