Articles
S-allele constitution of hexaploid European plum cultivars
Article number
1231_25
Pages
151 – 156
Language
English
Abstract
European plum (Prunus domestica) is an important fruit crop in Central Europe with a complex hexaploid genome of unknown origin.
The characterization of the S-locus of 17 economically important European plum cultivars was carried out.
To characterize the length polymorphism for first and second introns, the PaConsI and EM-PC primer pairs were used.
Altogether, 18 different alleles were identified, of which 11 S-RNase alleles were first detected.
To confirm S-allele identity, partial S-RNase sequences were determined and blasted on the GenBank database.
Partial S-haplotype-specific F-box alleles were also cloned and sequenced.
Several trans-specific alleles were detected and their phylogenetic analysis and Tajima’s D test showed the rate of evolution dramatically accelerates when allele specificity is modified by fixed amino acid replacements.
A total of 17 different S-genotypes were assigned, so the discrimination of all assayed plum cultivars was successful based on their unique S-genotypes.
Cross-incompatible cultivars were not found.
Our analysis is continued to identify mutations in the S-locus genes that might be responsible for the transition from a self-incompatible to a self-compatible phenotype in a hexaploid species.
The characterization of the S-locus of 17 economically important European plum cultivars was carried out.
To characterize the length polymorphism for first and second introns, the PaConsI and EM-PC primer pairs were used.
Altogether, 18 different alleles were identified, of which 11 S-RNase alleles were first detected.
To confirm S-allele identity, partial S-RNase sequences were determined and blasted on the GenBank database.
Partial S-haplotype-specific F-box alleles were also cloned and sequenced.
Several trans-specific alleles were detected and their phylogenetic analysis and Tajima’s D test showed the rate of evolution dramatically accelerates when allele specificity is modified by fixed amino acid replacements.
A total of 17 different S-genotypes were assigned, so the discrimination of all assayed plum cultivars was successful based on their unique S-genotypes.
Cross-incompatible cultivars were not found.
Our analysis is continued to identify mutations in the S-locus genes that might be responsible for the transition from a self-incompatible to a self-compatible phenotype in a hexaploid species.
Authors
N. Makovics-Zsohár, A. Hegedűs, J. Halász
Keywords
Prunus domestica, evolution, intron length polymorphism, S-genotype, S-RNase
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