S-genotyping of Hungarian sour cherry cultivars

Sour cherry (Prunus cerasus L.) is native to Hungary and some Hungarian cultivars are grown worldwide.
Sour cherry includes both self-incompatible and self-compatible accessions.
Being an allotetraploid spontaneous hybrid of sweet and ground cherries with 32 chromosomes in sporophytic cells, the S-allele pools of sweet and sour cherries are expected to overlap.
SC is primarily due to inactive S-haplotype-specific F-box (SFB) protein in diploid pollen carrying two non-functional S-haplotypes.
We determined the S-allele composition of 32 Hungarian sour cherry cultivars using intron length polymorphism, allele-specific PCR, CAPS markers and DNA sequencing.
We found a total of 10 S-haplotypes (S₁, S₄, S₆, S₉, S₁₂, S₁₃, S₁₄, S₂₆, S₃₅ and S₃₆) or their non-functional variants.
It was the first time to use the first intron length analysis for S-allele identification in sour cherry.
Both S-genotype and simple sequence repeat fingerprinting confirmed that ‘Pipacs 1’ and other famous amarelle-type sour cherries (‘Pándy’ and ‘Montmorency’) share no common ancestry. ‘Pipacs 1’ is a unique cherry cultivar exclusively accumulating considerable amounts of isoflavonoid compounds in fruit.
Hence its molecular differentiation from other amarelle-type cherries may be of great importance.

II International Workshop on Floral Biology and S-Incompatibility in Fruit Species

J. Halász, E. Balogh, N. Makovics-Zsohár, A. Hegedűs

CAPS, S-haplotype, fingerprinting, intron length polymorphism, Prunus cerasus

https://doi.org/10.17660/ActaHortic.2019.1231.27