Articles
Development of double-haploid lines in Galia-type melons (Cucumis melo var. cantalupensis) using in vitro colchicine treatments
Article number
1282_23
Pages
149 – 156
Language
English
Abstract
Melon is an important vegetable crop in the world.
Cantaloupe types of melons (Cucumis melo var. cantalupensis L.) ripens early and are aromatic but they have a short shelf life, which limits transport and export opportunities.
In this research, we used a total of 84 plants originating from BC3 populations to developed 100% homozygous haploid lines by using irradiated pollen (300 Grays of Cobalt60) technique for breeding cantaloupe melons with long shelf life.
A total of 600 haploid melon lines were obtained with irradiated pollen technique.
The haploid plants of these lines were propagated by in vitro micro-cuttings.
In vitro colchicine (0.5%) was applied to 15 micro-cuttings of each haploid line for 2 h to double the chromosome number.
After rinsing 3 times with sterile distilled water micro-cuttings were dried and transferred into test tubes contaning MS medium.
Afterwards they were placed in growth chamber having 24°C temperature and 16/8 h day/night photoperiod conditions.
After 1 month plants were acclimatized to greenhouse.
The ploidy level was identified by morphological observations, pollen presence and chloroplast/stoma counting.
Among the 600 in vitro colchicine applied haploid lines, 152 were found to be diploid and the success of in vitro duplication was obtained as 25.33%.
Cantaloupe types of melons (Cucumis melo var. cantalupensis L.) ripens early and are aromatic but they have a short shelf life, which limits transport and export opportunities.
In this research, we used a total of 84 plants originating from BC3 populations to developed 100% homozygous haploid lines by using irradiated pollen (300 Grays of Cobalt60) technique for breeding cantaloupe melons with long shelf life.
A total of 600 haploid melon lines were obtained with irradiated pollen technique.
The haploid plants of these lines were propagated by in vitro micro-cuttings.
In vitro colchicine (0.5%) was applied to 15 micro-cuttings of each haploid line for 2 h to double the chromosome number.
After rinsing 3 times with sterile distilled water micro-cuttings were dried and transferred into test tubes contaning MS medium.
Afterwards they were placed in growth chamber having 24°C temperature and 16/8 h day/night photoperiod conditions.
After 1 month plants were acclimatized to greenhouse.
The ploidy level was identified by morphological observations, pollen presence and chloroplast/stoma counting.
Among the 600 in vitro colchicine applied haploid lines, 152 were found to be diploid and the success of in vitro duplication was obtained as 25.33%.
Authors
S. Pamuk, N. Sari, I. Solmaz, I. Mancak
Keywords
melon, irradiated pollen, ploidy level, micro-cutting
Groups involved
- Division Plant Genetic Resources, Breeding and Biotechnology
- Division Vine and Berry Fruits
- Division Temperate Tree Fruits
- Division Tropical and Subtropical Fruit and Nuts
- Division Horticulture for Human Health
- Division Ornamental Plants
- Division Vegetables, Roots and Tubers
- Commission Banana
- Working Group Biotechnology of Horticultural Species
- Division Temperate Tree Nuts
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