Molecular cloning and expression analysis of the transcription factor gene ChCBF from hazelnut (Corylus heterophylla Fischer)

The CBF (C-repeat binding factor) genes are an important family of transcription factors in plants, encoding transcriptional regulators with a variety of functions involved in developmental and physiological processes.
In this study, the conserved domain of the CBF gene in Corylus heterophylla Fischer. was cloned using two degenerate primers, and the 5’terminal and 3’terminal of the gene were amplified by using rapid amplification of cDNA ends (RACE) methods.
The complete cDNA sequence termed as ChCBF1 was obtained and the sequence was submitted to GenBank (GenBank accession number HQ639413). Sequence analysis showed that the nucleotide sequence of ChCBF1 was 1053 bp, containing an open reading frame (ORF) of 684 bp and encoding a protein with 227 amino acids.
The alignment analysis indicated that the predicted protein sequence contained a typical AP2/EREBP DNA-binding domain.
Homology analysis showed that the ChCBF1 protein had high homology with CBF from Citrus trifoliata and Malus × domestica. ChCBF1 gene showed that its transcripts were abundant in floral bud.
Its hydrophobicity/hydrophilic,physico-chemical properties, phylogenetic tree, and main functional domains were predicted.
Real-time quantitative polymerase chain reaction (Real-Time PCR) analysis was performed for ChCBF1 treated with a low-temperature of 4°C. The results showed that ChCBF1 gene expression was increased 2 h after being cold induced and reached a peak after 4 h, and then decreased. ChCBF1 might play a key role during the development of cold resistance in C. heterophylla Fischer.

XXX International Horticultural Congress IHC2018: II International Symposium on Plant Breeding in Horticulture

X. Chen, W. Guo, L. Xu, L.S. Zhang, Q.Z. Liu

CBF gene, transcription factor, Corylus heterophylla, RACE, Real-Time PCR

https://doi.org/10.17660/ActaHortic.2020.1282.49