Analysis of the genetic diversity for the Juglans hopeiensis cultivars by FISH-AFLP markers

The Juglans hopeiensis cultivars were used to study the genetic diversity and parentage relationship in molecular level, providing scientific basis to protect and utilize the germplasm effectively.
FISH-AFLP markers were conducted, with 8 primers to analyze the genetic diversity and parentage relationship among 28 Juglans cultivars at genomic DNA level.
Totally 1009 AFLP fragments were obtained, in which there were 837 polymorphic bands and 202 characteristic bands.
The average percentage of polymorphic bands (PPB) was 82.95% and the genetic similarity coefficient distributed among 0.4446~0.744. The value of effective number of alleles (Ne) ranged from 1.00 to 1.99 with an average of 0.36±0.01; Nei’s gene diversity (H) ranged from 0.00 to 0.39 with an average of 0.23±0.01 and Shannon’s information index (I) ranged from 0.00 to 0.59 with an average of 0.44±0.02. The J. hopeiensis germplasm had abundant genetic diversity and complex genetic background.
Dendrogram of walnut materials origins based on AFLP data showed that ‘shaanxiguanmao’ was different, maybe new taxa.

XXX International Horticultural Congress IHC2018: II International Symposium on Plant Breeding in Horticulture

X. Chen, W. Guo, L. Xu, Q.Z. Liu

AFLP, J. hopeiensis, genetic diversity, cultivar identification, clustering analysis

https://doi.org/10.17660/ActaHortic.2020.1282.50