Identification of a negative MYB regulator gene for anthocyanin biosynthesis in peach fruit

Anthocyanin accumulation in fruit is beneficial for human health due to the anti-oxidation activity.
Here, we report the feedback regulation mechanism underlying anthocyanin accumulation in peach mesocarp.
In the blood-flesh ‘Dahongpao’ peach cultivar, the expression level of the positive PpMYB10.1 regulator increased during fruit developmental stages, and reached a peak at the ripening stage, which corresponded with changes in anthocyanin content.
Interestingly, another MYB gene, designated PpMYB6, was also highly expressed at fruit ripening stages.
Functional analysis showed that PpMYB6 could negatively regulate two anthocyanin structural genes, PpDFR and PpUFGT, in a dual luciferase assay and inhibited anthocyanin coloration when it was co-infiltrated with PpMYB10.1 in a transient color assay.
These results suggest that anthocyanin accumulation in blood-peach fruit may be regulated by a feedback loop comprising PpMYB10.1, PpMYB6 and anthocyanin structural genes.

IX International Peach Symposium

H. Zhou, Y. Han

Prunus persica, anthocyanin, repressor

https://doi.org/10.17660/ActaHortic.2021.1304.15