Articles
Applicability of a new sex-linked codominant DNA marker among asparagus cultivars and various Asparagus species
Article number
1376_12
Pages
75 – 80
Language
English
Abstract
Garden asparagus (Asparagus officinalis L.) is a dioecious species, with male [XY] and female [XX] individuals.
Male individuals are considered to be more desirable than females due to their higher yields, all-male cultivars have agronomic advantages over mixed-sex cultivars.
To reduce the time for asparagus breeding, various dominant and codominant male-specific DNA markers have been reported.
Codominant markers are preferable over dominant markers because codominant markers can discriminate between females [XX], males [XY] and supermales [YY] in A. officinalis. To date, two sex-linked co-dominant molecular markers Asp2-SP6 and RM17 have already been reported, although the applicability of these markers among various asparagus cultivars and Asparagus species was very little analyzed.
Recently, we developed a new CAPS marker (named SSM01), based on the sequence around RM17 marker.
Since SSM01 amplify ca. 470 bp fragment and only Y-specific sequence was digested into ca. 270/200 bp by XspI, this marker can discriminate between females [XX], males [XY] and supermales [YY] in A. officinalis. SSM01 is also applicable for discrimination between X- and Y-specific sequences in purple asparagus cultivars and three dioecious Asparagus species, A. kiusianus, A. pseudoscaber and A. maritimus. Interestingly, sequence analysis showed that the position of Y-specific XspI site are different among A. officinalis, purple asparagus and three related species, although each XspI site is Y-specific.
Our results indicated that SSM01 is very useful marker to identify X- and Y-specific sequences among asparagus cultivars and three Asparagus species.
Male individuals are considered to be more desirable than females due to their higher yields, all-male cultivars have agronomic advantages over mixed-sex cultivars.
To reduce the time for asparagus breeding, various dominant and codominant male-specific DNA markers have been reported.
Codominant markers are preferable over dominant markers because codominant markers can discriminate between females [XX], males [XY] and supermales [YY] in A. officinalis. To date, two sex-linked co-dominant molecular markers Asp2-SP6 and RM17 have already been reported, although the applicability of these markers among various asparagus cultivars and Asparagus species was very little analyzed.
Recently, we developed a new CAPS marker (named SSM01), based on the sequence around RM17 marker.
Since SSM01 amplify ca. 470 bp fragment and only Y-specific sequence was digested into ca. 270/200 bp by XspI, this marker can discriminate between females [XX], males [XY] and supermales [YY] in A. officinalis. SSM01 is also applicable for discrimination between X- and Y-specific sequences in purple asparagus cultivars and three dioecious Asparagus species, A. kiusianus, A. pseudoscaber and A. maritimus. Interestingly, sequence analysis showed that the position of Y-specific XspI site are different among A. officinalis, purple asparagus and three related species, although each XspI site is Y-specific.
Our results indicated that SSM01 is very useful marker to identify X- and Y-specific sequences among asparagus cultivars and three Asparagus species.
Publication
Authors
M. Akahori, Y. Sato, A. Kanno
Keywords
codominant marker, CAPS marker, supermale, Asparagus species
Groups involved
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