Articles
Asparagus breeding by anther culture of an interspecific hybrid (A. officinalis × A. kiusianus)
Article number
1376_4
Pages
23 – 30
Language
English
Abstract
Although asparagus (Asparagus officinalis) shows varietal difference of color and size of spears, the range of variation is limited in many traits because of the breeding history of the crop with limited strains. Asparagus kiusianus, an endemic species in Japan, has different plant form and is expected to have some abiotic stress tolerance because of being native to the coast.
Since the species is cross compatible with asparagus, it would be possible to use it as breeding material.
In this study, it is attempted to obtain homozygous pure line plants derived from interspecific hybrids by means of anther culture.
Anthers collected at different times were used to investigate the effect of gum Arabic in the medium on callus and shoot formation.
It was shown that there was no difference of callus and shoot formation between the concentrations of gum Arabic, but the response differed depending on the sampling time of anthers.
Namely, the values of callus and shoot formation from anthers collected in April and June tended to be higher than those collected in March.
Genotyping of 607 regenerated plants with RM17 markers proved that four females and eight supermales could be obtained.
Screening of ploidy levels of the females and supermales with flow cytometry exhibited that four females and seven supermales are diploids and tetraploids, respectively.
One supermale died before the screening.
These results indicated that pure homozygous lines can be established by anther culture even in interspecific hybrid individuals between asparagus and A. kiusianus, suggesting the possibility of future expansion of the variation of genetic traits in the next generation of asparagus breeding.
Since the species is cross compatible with asparagus, it would be possible to use it as breeding material.
In this study, it is attempted to obtain homozygous pure line plants derived from interspecific hybrids by means of anther culture.
Anthers collected at different times were used to investigate the effect of gum Arabic in the medium on callus and shoot formation.
It was shown that there was no difference of callus and shoot formation between the concentrations of gum Arabic, but the response differed depending on the sampling time of anthers.
Namely, the values of callus and shoot formation from anthers collected in April and June tended to be higher than those collected in March.
Genotyping of 607 regenerated plants with RM17 markers proved that four females and eight supermales could be obtained.
Screening of ploidy levels of the females and supermales with flow cytometry exhibited that four females and seven supermales are diploids and tetraploids, respectively.
One supermale died before the screening.
These results indicated that pure homozygous lines can be established by anther culture even in interspecific hybrid individuals between asparagus and A. kiusianus, suggesting the possibility of future expansion of the variation of genetic traits in the next generation of asparagus breeding.
Publication
Authors
R. Nakagawa, E. Kato, N. Iyama, N. Higuchi, R. Kamei, C. Kurihara, H. Yamada, Y. Mizunoe, Y. Ozaki
Keywords
androgenesis, DNA marker, doubled haploids, flow cytometry, interspecific hybrid
Groups involved
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