Articles
Unveiling the effects of hinokitiol (β-thujaplicin) on ergosterol biosynthesis in Botrytis cinerea
Article number
1378_17
Pages
125 – 132
Language
English
Abstract
After harvest, the shelf life of the fresh postharvest product will be shorter if decay caused by fungi is not controlled.
Gray mold is one of the major postharvest diseases caused by Botrytis cinerea, leading to postharvest losses.
In this study, hinokitiol, also known as β- thujaplicin, was selected as a natural fungistatic substance.
Hinokitiol has potential for antimicrobial, antiviral, insecticidal, and antitumor activity.
It is reported that hinokitiol had the ability to inhibit the growth of such fungi with low mammalian toxicity as an alternative to synthetic fungicides.
The present study aims to investigate the antifungal activity of hinokitiol against pathogens as well as the underlying mechanism involved. B. cinerea isolated from strawberries fruit was use.
The result shows that the mycelial growth of B. cinerea on potato dextrose agar (PDA) was strongly inhibited by hinokitiol.
The colony diameters decreased along with the increase of hinokitiol doses.
According to the observation during 72 h, the inhibitory effects depended on concentration and time.
Spore germination and germ tube elongation of B. cinerea were similarly considerably suppressed by hinokitiol dosages, which were positively associated.
After the incubation of spores in potato dextrose broth (PDB) at 25°C, ergosterol level of B. cinerea treated with hinokitiol remained constant whereas that in control increased.
This phenomenon will lead to the investigation of the action mechanism of the ergosterol biosynthesis pathway by determining the effect of hinokitiols application on the expression of genes related to ergosterol biosynthesis.
Gray mold is one of the major postharvest diseases caused by Botrytis cinerea, leading to postharvest losses.
In this study, hinokitiol, also known as β- thujaplicin, was selected as a natural fungistatic substance.
Hinokitiol has potential for antimicrobial, antiviral, insecticidal, and antitumor activity.
It is reported that hinokitiol had the ability to inhibit the growth of such fungi with low mammalian toxicity as an alternative to synthetic fungicides.
The present study aims to investigate the antifungal activity of hinokitiol against pathogens as well as the underlying mechanism involved. B. cinerea isolated from strawberries fruit was use.
The result shows that the mycelial growth of B. cinerea on potato dextrose agar (PDA) was strongly inhibited by hinokitiol.
The colony diameters decreased along with the increase of hinokitiol doses.
According to the observation during 72 h, the inhibitory effects depended on concentration and time.
Spore germination and germ tube elongation of B. cinerea were similarly considerably suppressed by hinokitiol dosages, which were positively associated.
After the incubation of spores in potato dextrose broth (PDB) at 25°C, ergosterol level of B. cinerea treated with hinokitiol remained constant whereas that in control increased.
This phenomenon will lead to the investigation of the action mechanism of the ergosterol biosynthesis pathway by determining the effect of hinokitiols application on the expression of genes related to ergosterol biosynthesis.
Authors
N. Benyakart, H. Umehara, D. Ciptaningtyas, T. Shiina
Keywords
Botrytis cinerea, ergosterol biosynthesis, hinokitiol, SEM
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