Articles
Application of a bio-control agent and other practices for controlling strawberry anthracnose in Taiwan
Article number
1378_4
Pages
23 – 32
Language
English
Abstract
Strawberry (Fragaria × ananassa Duchesne) is an economically important crop in Taiwan, however, because of the outbreak of anthracnose in early 2010, the short supply of seedlings became a serious problem for strawberry production.
The isolates of anthracnose causing organisms were collected from major strawberry cultivation areas in Miaoli County, and mainly identified as Colletotrichum siamense. These isolates were proved to be pathogenic to the leaves, crown and roots of the plant.
The bio-control agent Bacillus amyloliquefaciens P-2-2, was isolated from a rice paddy field and showed significant inhibition on many fungal pathogens of strawberry.
The seedlings used in this study were treated before transplanting with 400-fold dilution of B. amyloliquefaciens P-2-2 fermentation broth every two weeks to protect the crown of the seedlings from anthracnose infection.
These seedlings were also sprayed with the hypochlorous acid solution daily to prevent the spread of pathogen spores.
Sterilization of soil by biological soil disinfestation (BSD) method was done during field preparation.
After transplanting the seedlings were treated with B. amyloliquefaciens P-2-2 until the end of the growing season.
The results of the field test showed that the survival rate of the untreated group in demonstration area of Dahu Township was 83.9%, whereas that of healthy strawberry seedlings treated with B. amyloliquefaciens P-2-2 increased to 95.1%. The replanting rate of strawberry in the untreated group area was 37.6%, whereas the B. amyloliquefaciens P-2-2 treated area was reduced significantly to 10.7%. The results indicated that application of a bio-control agent with other practices were effective for controlling strawberry anthracnose.
The isolates of anthracnose causing organisms were collected from major strawberry cultivation areas in Miaoli County, and mainly identified as Colletotrichum siamense. These isolates were proved to be pathogenic to the leaves, crown and roots of the plant.
The bio-control agent Bacillus amyloliquefaciens P-2-2, was isolated from a rice paddy field and showed significant inhibition on many fungal pathogens of strawberry.
The seedlings used in this study were treated before transplanting with 400-fold dilution of B. amyloliquefaciens P-2-2 fermentation broth every two weeks to protect the crown of the seedlings from anthracnose infection.
These seedlings were also sprayed with the hypochlorous acid solution daily to prevent the spread of pathogen spores.
Sterilization of soil by biological soil disinfestation (BSD) method was done during field preparation.
After transplanting the seedlings were treated with B. amyloliquefaciens P-2-2 until the end of the growing season.
The results of the field test showed that the survival rate of the untreated group in demonstration area of Dahu Township was 83.9%, whereas that of healthy strawberry seedlings treated with B. amyloliquefaciens P-2-2 increased to 95.1%. The replanting rate of strawberry in the untreated group area was 37.6%, whereas the B. amyloliquefaciens P-2-2 treated area was reduced significantly to 10.7%. The results indicated that application of a bio-control agent with other practices were effective for controlling strawberry anthracnose.
Authors
T.C. Lin, C.S. You, I.S. Wu, C.H. Tsai, J.N. Tsai, J.Z. Yu, R.J. Chang
Keywords
Taiwan, strawberry, anthracnose, bio-control agent, biological soil disinfestation
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