Articles
Phenotypical and biochemical evaluation of the Sideritis scardica, hyssopifolia, syriaca and raeseri genotypes acclimated in Romania
Article number
1387_39
Pages
283 – 290
Language
English
Abstract
The interest and demand for new species appreciated for medicinal and food purposes has steadily developed in Romania.
Therefore, PGRB Buzau researchers focused on the acclimatization of new species, succeeding in the acclimatization of over 50 new species that have not been cultivated in Romania so far, releasing even new cultivars.
Special attention was paid to the species of Sideritis, which were gradually introduced into the study starting from 2004. The genus Sideritis has over 150 species endemically dispersed in a wide area, including the Balkan area close to Romania, such as Bulgaria, Macedonia, Greece, Cyprus and going further up to Spain and Portugal.
The research undertaken ended with the acclimatization and patenting of a new cultivar of Sideritis scardica registered in the Official Catalog of Cultivated Plants in Romania under the name of Domnesc. In terms of phenotypic expressiveness, different values were recorded that give biometric and phenological distinction to the cultivars.
For instance, Sideritis hyssopifolia presents inflorescences from the first year of cultivation, while in the 2nd,3rd and 4th year Sideritis scardica stands out as the species with the richest leaf mass and largest number of inflorescences per plant. Sideritis syriaca proved to be the species with the most intense aroma.
Further on, the varieties were also characterized by the volatile compounds content using the GC-MS technique, by the antioxidant activity using the DPPH method and the total content of polyphenols by the Folin-Ciocâlteu method.
In both cases it was observed that the total content of polyphenols and the antioxidant activity slightly vary from one variety to another (14.97-15.79 mg GAE g‑1 DM for TPC, and 202.36-241.05 mg TE g‑1 DM). The biggest variation was observed for the content of volatile compounds, among which the most important are α-pinene, β-pinene and α-bisabolol, that differed depending on the species.
Therefore, PGRB Buzau researchers focused on the acclimatization of new species, succeeding in the acclimatization of over 50 new species that have not been cultivated in Romania so far, releasing even new cultivars.
Special attention was paid to the species of Sideritis, which were gradually introduced into the study starting from 2004. The genus Sideritis has over 150 species endemically dispersed in a wide area, including the Balkan area close to Romania, such as Bulgaria, Macedonia, Greece, Cyprus and going further up to Spain and Portugal.
The research undertaken ended with the acclimatization and patenting of a new cultivar of Sideritis scardica registered in the Official Catalog of Cultivated Plants in Romania under the name of Domnesc. In terms of phenotypic expressiveness, different values were recorded that give biometric and phenological distinction to the cultivars.
For instance, Sideritis hyssopifolia presents inflorescences from the first year of cultivation, while in the 2nd,3rd and 4th year Sideritis scardica stands out as the species with the richest leaf mass and largest number of inflorescences per plant. Sideritis syriaca proved to be the species with the most intense aroma.
Further on, the varieties were also characterized by the volatile compounds content using the GC-MS technique, by the antioxidant activity using the DPPH method and the total content of polyphenols by the Folin-Ciocâlteu method.
In both cases it was observed that the total content of polyphenols and the antioxidant activity slightly vary from one variety to another (14.97-15.79 mg GAE g‑1 DM for TPC, and 202.36-241.05 mg TE g‑1 DM). The biggest variation was observed for the content of volatile compounds, among which the most important are α-pinene, β-pinene and α-bisabolol, that differed depending on the species.
Publication
Authors
G. Negoșanu, C. Vînătoru, V. Ion, A. Dîcă, A. Asănică
Keywords
breeding, medicinal purposes, phenotypic expressiveness, antioxidant activity, volatile compounds
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