Articles
Determination of antioxidant activity of olive oil cultivars in the region of Epirus, Greece
Article number
1407_13
Pages
99 – 106
Language
English
Abstract
Total phenolic content entails a lot of substances acting together.
They are known for their beneficial antioxidant potential, their substantial role in the mechanism of stress tolerance and the organoleptic properties they confer to the oil.
The aim of this study was to determine the antioxidant activity of polar extracts derived from monocultivar olive oils from the Epirus region in Greece, using spectrophotometry, in the crop year 2021-2022. The studied cultivars were indigenous, including cultivars ‘Konservolia’, ‘Lianolia Prevezas’, and ‘Chondrolia Igoumenitsas’, or introduced from other geographic regions of Greece (‘Koroneiki’ and ‘Kalamon’). Extraction of total phenolics was performed using liquid-liquid extraction (LLE) with an extraction solvent mixture of methanol:water 80:20 and a hexane step for removing non-polar oily fractions of oil.
Total phenolics (TP), which were determined using Folin-Ciocalteu reagent, ranged from 278 to 498 mg gallic acid equivalent (GAE) kg‑1 olive oil.
Total flavonoid content (TFC) using the aluminium chloride method expressed as mg quercetin equivalents (QE) kg‑1, was between 232 to 941 mg QE kg‑1 olive oil.
Ortho-diphenols determined with sodium molybdate, ranged from 47 to 176 mg GAE kg‑1 olive oil.
Radical scavenging activity towards specific radicals, namely 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), were also determined.
These were linearly correlated with oil mass (R2 0.93-0.99) and the volume of hydrophilic extracts (0.74-0.96), respectively.
They are known for their beneficial antioxidant potential, their substantial role in the mechanism of stress tolerance and the organoleptic properties they confer to the oil.
The aim of this study was to determine the antioxidant activity of polar extracts derived from monocultivar olive oils from the Epirus region in Greece, using spectrophotometry, in the crop year 2021-2022. The studied cultivars were indigenous, including cultivars ‘Konservolia’, ‘Lianolia Prevezas’, and ‘Chondrolia Igoumenitsas’, or introduced from other geographic regions of Greece (‘Koroneiki’ and ‘Kalamon’). Extraction of total phenolics was performed using liquid-liquid extraction (LLE) with an extraction solvent mixture of methanol:water 80:20 and a hexane step for removing non-polar oily fractions of oil.
Total phenolics (TP), which were determined using Folin-Ciocalteu reagent, ranged from 278 to 498 mg gallic acid equivalent (GAE) kg‑1 olive oil.
Total flavonoid content (TFC) using the aluminium chloride method expressed as mg quercetin equivalents (QE) kg‑1, was between 232 to 941 mg QE kg‑1 olive oil.
Ortho-diphenols determined with sodium molybdate, ranged from 47 to 176 mg GAE kg‑1 olive oil.
Radical scavenging activity towards specific radicals, namely 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), were also determined.
These were linearly correlated with oil mass (R2 0.93-0.99) and the volume of hydrophilic extracts (0.74-0.96), respectively.
Authors
N. Vitsiou, C. Karipidis, V. Stournaras, P. Mpeza
Keywords
local olive tree cultivars, total phenols, o-diphenols, flavonoids, antiradical activity
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