Articles
Obtaining protein isolates from quinoa (Chenopodium quinoa ‘Bianca de Junin’) for use in high protein supplements
Article number
1407_15
Pages
115 – 120
Language
English
Abstract
Proteins are compounds that act in a broad range of bodily functions, including the formation of bones, ligaments and tendons and muscle contraction, among others.
Proteins are made up of 20 amino acids, where 9 amino acids are essential because they cannot be biosynthesized by humans.
Proteins with an animal origin stand out because they have a high biological value, supplying all essential amino acids; however, the cost can be high, limiting consumption in low-income populations.
Quinoa is a pseudocereal that is highly valued by the FAO as an ally in the fight against hunger, poverty, and malnutrition, problems that affect two thirds of the global population.
Its high protein content with all essential amino acids, especially histidine and lysine (limiting amino acids in cereals), offers an alternative to animal protein that can be used to prevent malnutrition in children.
The present study aimed to obtain protein isolates from quinoa (Chenopodium quinoa ‘Bianca de Junin’) grown in Boyacá, Colombia using 4 methods for use in protein supplements.
The extraction process was carried out by applying: 1) enzymatic methods to pre-cooked quinoa flour degreased with petroleum ether, 2) isoelectric precipitation in pre-cooked quinoa flour degreased with petroleum ether, 3) isoelectric precipitation and ultrasound applications in destarched quinoa flour, and 4) isoelectric precipitation in pre-cooked, destarched quinoa flour.
The method that obtained the highest protein concentration was the third method, with a concentration of 72.4%, which is recommended for use in food since the use of solvents can leave residues that are not favorable for consumer health.
Supplements made with this protein isolate can be used for patients with malnutrition and for athletes, among others.
Proteins are made up of 20 amino acids, where 9 amino acids are essential because they cannot be biosynthesized by humans.
Proteins with an animal origin stand out because they have a high biological value, supplying all essential amino acids; however, the cost can be high, limiting consumption in low-income populations.
Quinoa is a pseudocereal that is highly valued by the FAO as an ally in the fight against hunger, poverty, and malnutrition, problems that affect two thirds of the global population.
Its high protein content with all essential amino acids, especially histidine and lysine (limiting amino acids in cereals), offers an alternative to animal protein that can be used to prevent malnutrition in children.
The present study aimed to obtain protein isolates from quinoa (Chenopodium quinoa ‘Bianca de Junin’) grown in Boyacá, Colombia using 4 methods for use in protein supplements.
The extraction process was carried out by applying: 1) enzymatic methods to pre-cooked quinoa flour degreased with petroleum ether, 2) isoelectric precipitation in pre-cooked quinoa flour degreased with petroleum ether, 3) isoelectric precipitation and ultrasound applications in destarched quinoa flour, and 4) isoelectric precipitation in pre-cooked, destarched quinoa flour.
The method that obtained the highest protein concentration was the third method, with a concentration of 72.4%, which is recommended for use in food since the use of solvents can leave residues that are not favorable for consumer health.
Supplements made with this protein isolate can be used for patients with malnutrition and for athletes, among others.
Authors
C. Gutierrez P., M.S. Hernandez, M.C. Lares, J.L. Sandoval S.
Keywords
malnutrition, supplements, isoelectric precipitation, amino acids, starch, degreasing, protein extraction
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