Articles
Temperature and short-term waterlogging impact the degradation of extracted plant pigments from kale (Brassica oleracea L.)
Article number
1407_5
Pages
33 – 42
Language
English
Abstract
Current predictions associated with climate change anticipate greater frequencies of precipitation events, with greater quantities of precipitation.
Previous research has demonstrated that waterlogging of plants causes reductions in plant pigments.
However, the stability of plant pigment extracts has yet to be quantified.
The current study investigated whether waterlogging and different storage temperatures of extracted samples would impact the degradation rate of plant pigments of kale.
Carotenoid and chlorophylls were extracted from kale plants that were waterlogged or non-waterlogged, stored in acetone, and sealed in amber vials.
Vials were stored at four different temperatures (25, 10, -10, and -80°C) and analyzed 0, 30, 60, 120, and 240 days after initial extraction.
After 240 days of storage, all pigments exhibited a treatment by temperature by day interaction.
Overall, as storage temperature decreased, the rate of degradation decreased, with waterlogged kale plants having a lower concentration of pigments compared to non-waterlogged plants.
Additionally, greater variability arose in individual pigment retention like that of chlorophyll b.
Chlorophyll b concentrations of waterlogged plants had greater retention at 10 and 25°C than that of non-waterlogged plants at the same temperatures measured after 30 days of storage.
Our findings highlight the complexity of pigment stability under varying abiotic stressor and storage conditions, emphasizing the need for rapid analysis post-extraction to maintain nutritional quality.
Further, research is needed to explore the role of stress-induced metabolic changes in pigment stability and their implications for the retention of phytonutrients in stored plant products.
Previous research has demonstrated that waterlogging of plants causes reductions in plant pigments.
However, the stability of plant pigment extracts has yet to be quantified.
The current study investigated whether waterlogging and different storage temperatures of extracted samples would impact the degradation rate of plant pigments of kale.
Carotenoid and chlorophylls were extracted from kale plants that were waterlogged or non-waterlogged, stored in acetone, and sealed in amber vials.
Vials were stored at four different temperatures (25, 10, -10, and -80°C) and analyzed 0, 30, 60, 120, and 240 days after initial extraction.
After 240 days of storage, all pigments exhibited a treatment by temperature by day interaction.
Overall, as storage temperature decreased, the rate of degradation decreased, with waterlogged kale plants having a lower concentration of pigments compared to non-waterlogged plants.
Additionally, greater variability arose in individual pigment retention like that of chlorophyll b.
Chlorophyll b concentrations of waterlogged plants had greater retention at 10 and 25°C than that of non-waterlogged plants at the same temperatures measured after 30 days of storage.
Our findings highlight the complexity of pigment stability under varying abiotic stressor and storage conditions, emphasizing the need for rapid analysis post-extraction to maintain nutritional quality.
Further, research is needed to explore the role of stress-induced metabolic changes in pigment stability and their implications for the retention of phytonutrients in stored plant products.
Authors
S.R. Brazel, B. Adhikari, T.C. Barickman
Keywords
kale, carotenoids, chlorophyll, abiotic stress, climate change
Online Articles (17)