Articles
Preliminary results regarding the in vitro propagation of Actinidia arguta ‘Vip Red’
Article number
1444_35
Pages
261 – 268
Language
English
Abstract
Over the past 70 years, kiwifruit (Actinidia sp.) has gained high popularity worldwide, due to its recognized nutritional and medicinal properties.
To support the increasing demand for planting materials, many countries are employing tissue culture techniques as alternative methods for plant propagation.
This preliminary study offers insights into how, the newly introduced cultivar ‘Vip Red’ in Romania, responds to different culture media with various types and concentrations of growth regulators.
Experiments involving NaDCC sterilized explants showed a remarkable 100% survival rate, highlighting its effectiveness in plant tissue culture.
The study reveals that the composition of the growth medium significantly influences shoot number, root development, and callus formation.
Adjustments to nitrogen and calcium sources, such as doubling NH4NO3 concentration and replacing Ca(NO3)2 with CaCl2, notably enhance multiplication rates and root numbers, emphasizing the importance of these elements in plant growth.
However, manipulating the concentration of plant growth regulators like BAP and IAA had a complex impact on development: reducing their concentrations increased shoot numbers but decreased shoot size, while high concentrations inhibited shoot growth and callus tissue development.
To support the increasing demand for planting materials, many countries are employing tissue culture techniques as alternative methods for plant propagation.
This preliminary study offers insights into how, the newly introduced cultivar ‘Vip Red’ in Romania, responds to different culture media with various types and concentrations of growth regulators.
Experiments involving NaDCC sterilized explants showed a remarkable 100% survival rate, highlighting its effectiveness in plant tissue culture.
The study reveals that the composition of the growth medium significantly influences shoot number, root development, and callus formation.
Adjustments to nitrogen and calcium sources, such as doubling NH4NO3 concentration and replacing Ca(NO3)2 with CaCl2, notably enhance multiplication rates and root numbers, emphasizing the importance of these elements in plant growth.
However, manipulating the concentration of plant growth regulators like BAP and IAA had a complex impact on development: reducing their concentrations increased shoot numbers but decreased shoot size, while high concentrations inhibited shoot growth and callus tissue development.
Authors
T. Hriban, O. Venat, I.C. Nicolae, A. Peticila, F. Stănică
Keywords
Actinidia sp., explants, culture media, growth regulators, NaDCC
Groups involved
- Division Vine and Berry Fruits
- Division Plant-Environment Interactions in Field Systems
- Division Horticulture for Human Health
- Working Group Vaccinium Species and Management
- Working Group Rubus and Ribes Species and Management
- Working Group Kiwifruit Culture and Management
- Working Group Strawberry Culture and Management
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