Articles
Optimization of an in vitro regeneration protocol to induce organogenesis in four cultivars of Fragaria × ananassa
Article number
1444_39
Pages
291 – 298
Language
English
Abstract
The cultivated strawberry (Fragaria × ananassa) is one of the most appreciated and popular fruits, and it is an allo-octoploid.
Due to its complex genome and high heterozygosity, improving specific traits through classical breeding methods becomes very challenging.
Therefore, it is essential to develop efficient in vitro regeneration protocols that are useful for the subsequent application of biotechnological tools aimed at the genetic improvement of selected strawberry cultivars.
In this study, the optimization of an in vitro regeneration protocol for four different strawberry (Fragaria × ananassa) cultivars, ‘Koinè’, ‘Shayda’, ‘Sibilla’, and ‘Murano’, was carried out.
In vitro strawberry leaves were utilized as starting explants, which were injured on the abaxial surface to induce the regeneration process.
Two different regeneration media were tested, based on MS salts and vitamins, which were supplemented with TDZ at 0.5 mg L-1 plus 2,4-D at 0.02 mg L-1 (medium S1), or with BAP at 3 mg L-1 plus IBA at 0.2 mg L‑1 (medium S2). The effect of leaf orientation (whether the abaxial surface is in contact with or not the medium) on regeneration efficiency was also investigated.
Results showed that medium S1 yielded the highest number of regenerated adventitious shoots per leaf compared to medium S2, with notable differences among the various genotypes.
Indeed, the regeneration percentage in S1 was around 85% for ‘Koinè’, 50% for ‘Shayda’, 35% for ‘Sibilla’, and 22.5% for ‘Murano’. The orientation of the leaves on the medium influenced the regeneration efficiency only for some of the cultivars tested, with better results when the injured explant was in contact with the medium.
The findings from this study will be exploited for future genetic engineering experiments on the cultivated strawberry.
Due to its complex genome and high heterozygosity, improving specific traits through classical breeding methods becomes very challenging.
Therefore, it is essential to develop efficient in vitro regeneration protocols that are useful for the subsequent application of biotechnological tools aimed at the genetic improvement of selected strawberry cultivars.
In this study, the optimization of an in vitro regeneration protocol for four different strawberry (Fragaria × ananassa) cultivars, ‘Koinè’, ‘Shayda’, ‘Sibilla’, and ‘Murano’, was carried out.
In vitro strawberry leaves were utilized as starting explants, which were injured on the abaxial surface to induce the regeneration process.
Two different regeneration media were tested, based on MS salts and vitamins, which were supplemented with TDZ at 0.5 mg L-1 plus 2,4-D at 0.02 mg L-1 (medium S1), or with BAP at 3 mg L-1 plus IBA at 0.2 mg L‑1 (medium S2). The effect of leaf orientation (whether the abaxial surface is in contact with or not the medium) on regeneration efficiency was also investigated.
Results showed that medium S1 yielded the highest number of regenerated adventitious shoots per leaf compared to medium S2, with notable differences among the various genotypes.
Indeed, the regeneration percentage in S1 was around 85% for ‘Koinè’, 50% for ‘Shayda’, 35% for ‘Sibilla’, and 22.5% for ‘Murano’. The orientation of the leaves on the medium influenced the regeneration efficiency only for some of the cultivars tested, with better results when the injured explant was in contact with the medium.
The findings from this study will be exploited for future genetic engineering experiments on the cultivated strawberry.
Authors
I. Piunti, L. Capriotti, B. Mezzetti, M. Zaccarini, M. Mattioli, S. Sabbadini
Keywords
leaf orientation, biotechnological approaches, genetic engineering, regeneration efficiency, adventitious shoots
Groups involved
- Division Vine and Berry Fruits
- Division Plant-Environment Interactions in Field Systems
- Division Horticulture for Human Health
- Working Group Vaccinium Species and Management
- Working Group Rubus and Ribes Species and Management
- Working Group Kiwifruit Culture and Management
- Working Group Strawberry Culture and Management
Online Articles (43)