Articles
Alternaria toxins: risk prevention and control for tomato food chain
Article number
1445_38
Pages
271 – 278
Language
English
Abstract
One of the major fungal pathogens of tomato fruits is Alternaria, developing during cultivation or post-harvest. Alternaria genus produce a wide range of toxins, and some are a cause for concern for consumer health, so named mycotoxins.
Recent scientific studies have led to the establishment of a European recommendation on the control of 3 Alternaria toxins (Alternariol, Alternariol monomethylether, Tenuazonic acid) of concern in foods, including processed tomato products.
To enrich existing data on the subject and to propose biocontrol strategies, Tomalt research project started.
Epidermal microbiota of processing tomato fruits was studied and confirmed the presence of Alternaria spp. Alternaria species were isolated and identified from contaminated tomatoes, and their toxinogenesis on different matrix in vitro was evaluated with quantification of 5 Alternaria toxins by LC-MS/MS, the 3 concerned by the recommendation plus Tentoxin and Altertoxin I. It highlights the importance of the strain chemotype and the growth matrix on mycotoxin contamination profile.
To better understand the fate of Alternaria toxins during tomato processing, naturally contaminated tomato fruits were processed into concentrates, and the impact of different processes on Alternaria toxins was investigated.
To combat Alternaria contamination of tomatoes, biocontrol solutions were tested.
A plant extract and Bacillus sp. group amyloliquefaciens isolated from processing tomato fruit showed promising antifungal effect against A. alternata.
Recent scientific studies have led to the establishment of a European recommendation on the control of 3 Alternaria toxins (Alternariol, Alternariol monomethylether, Tenuazonic acid) of concern in foods, including processed tomato products.
To enrich existing data on the subject and to propose biocontrol strategies, Tomalt research project started.
Epidermal microbiota of processing tomato fruits was studied and confirmed the presence of Alternaria spp. Alternaria species were isolated and identified from contaminated tomatoes, and their toxinogenesis on different matrix in vitro was evaluated with quantification of 5 Alternaria toxins by LC-MS/MS, the 3 concerned by the recommendation plus Tentoxin and Altertoxin I. It highlights the importance of the strain chemotype and the growth matrix on mycotoxin contamination profile.
To better understand the fate of Alternaria toxins during tomato processing, naturally contaminated tomato fruits were processed into concentrates, and the impact of different processes on Alternaria toxins was investigated.
To combat Alternaria contamination of tomatoes, biocontrol solutions were tested.
A plant extract and Bacillus sp. group amyloliquefaciens isolated from processing tomato fruit showed promising antifungal effect against A. alternata.
Authors
L. Dole, V. Chochois, N. Durand, A. Fontana, C. Strub, M. Bénas, R. Giovinazzo, F. Zuber, S. Schorr-Galindo
Keywords
Alternaria, mycotoxins, tomato, LC-MS/MS, metabarcoding, biocontrol
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