Articles
Valorization of tomato pomace to use it as a substrate in the fermentation
Article number
1445_41
Pages
295 – 302
Language
English
Abstract
This study investigated the hydrolysis of tomato pomace to obtain reducing sugar for subsequent fermentation and production of value-added products.
Three methods were employed: heat treated dilute acid hydrolysis, alkaline-assisted enzyme hydrolysis, and hydrothermal assisted enzyme hydrolysis.
To compare the effects of acid and alkaline pretreatments, 10% (w/v) tomato pomace suspensions were treated with either H2SO4 (0, 1, 2% w/v) for acid hydrolysis or NaOH (0, 1, 2% w/v) for alkaline pretreatment, both conducted at 121°C for 15 and 30 min.
Alkaline pretreated samples then underwent enzymatic hydrolysis at pH 5.0, 50°C, and 200 rpm for 18 h with the addition of pectinase and cellulase.
The DNS method was used to analyze the reducing sugar content.
Hydrothermal pretreatment for 15 min followed by enzymatic hydrolysis yielded the highest reducing sugar concentration (30.98 g L‑1) with 63.72% biomass conversion.
Hydrothermal pretreatment for 30 min, which is a chemical-free approach, combined with enzyme hydrolysis yielded a similar reducing sugar concentration (29.78 g L‑1) and biomass conversion (53.77%). Acid hydrolysis gave lower reducing sugar concentration and biomass conversion.
Three methods were employed: heat treated dilute acid hydrolysis, alkaline-assisted enzyme hydrolysis, and hydrothermal assisted enzyme hydrolysis.
To compare the effects of acid and alkaline pretreatments, 10% (w/v) tomato pomace suspensions were treated with either H2SO4 (0, 1, 2% w/v) for acid hydrolysis or NaOH (0, 1, 2% w/v) for alkaline pretreatment, both conducted at 121°C for 15 and 30 min.
Alkaline pretreated samples then underwent enzymatic hydrolysis at pH 5.0, 50°C, and 200 rpm for 18 h with the addition of pectinase and cellulase.
The DNS method was used to analyze the reducing sugar content.
Hydrothermal pretreatment for 15 min followed by enzymatic hydrolysis yielded the highest reducing sugar concentration (30.98 g L‑1) with 63.72% biomass conversion.
Hydrothermal pretreatment for 30 min, which is a chemical-free approach, combined with enzyme hydrolysis yielded a similar reducing sugar concentration (29.78 g L‑1) and biomass conversion (53.77%). Acid hydrolysis gave lower reducing sugar concentration and biomass conversion.
Authors
A.S. Akgun, D. Mentes, M.H. Öztop
Keywords
tomato pomace, hydrolysis, reducing sugar, waste management, sustainability
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