Articles
Mitigation of high temperature stress by foliar application of chitosan in tomato seedlings
Article number
1445_45
Pages
323 – 330
Language
English
Abstract
Chitosan is a natural biopolymer, non-toxic, biodegradable, and biocompatible.
It is derived from chitins and has the ability to improve physiological responses and reduce the negative impact of abiotic stresses in plants.
In order to study the impact of foliar application of chitosan on tomato seedling ‘Zucchero F1’ under high temperature stress, a factorial experiment was conducted under controlled environmental condition.
Treatments included two temperature levels (25/20°C optimal, 45/20°C high) and two different chitosan concentrations (100 and 150 mg L‑1 and 0 as control) with four replications.
Seedlings at the fourth true leaf stage were sprayed with different concentrations of chitosan or distilled water depending on the treatments, every two days.
After one week of chitosan application, half of the seedlings were subjected to high temperature stress at 45°C for 4 h each day.
The findings indicated a significant reduction in SPAD values and total chlorophyll concentrations in seedlings leaves subjected to high temperature condition.
However, the application of 100 mg L‑1 of chitosan resulted in an improvement in SPAD values whereas both dosages of chitosan led to an increase in total chlorophyll concentrations under stressful conditions.
Shoot length decreased under high temperatures and chitosan had no significant effect on shoot length in both stressed and non-stressed conditions.
Leaf relative water content significantly decreased in seedlings grown under heat stress and chitosan 100 mg L‑1 mitigated the adverse effects of it.
Overall, 100 mg L‑1 of chitosan can alleviate the adverse effect of high temperature stress in tomato seedlings.
It is derived from chitins and has the ability to improve physiological responses and reduce the negative impact of abiotic stresses in plants.
In order to study the impact of foliar application of chitosan on tomato seedling ‘Zucchero F1’ under high temperature stress, a factorial experiment was conducted under controlled environmental condition.
Treatments included two temperature levels (25/20°C optimal, 45/20°C high) and two different chitosan concentrations (100 and 150 mg L‑1 and 0 as control) with four replications.
Seedlings at the fourth true leaf stage were sprayed with different concentrations of chitosan or distilled water depending on the treatments, every two days.
After one week of chitosan application, half of the seedlings were subjected to high temperature stress at 45°C for 4 h each day.
The findings indicated a significant reduction in SPAD values and total chlorophyll concentrations in seedlings leaves subjected to high temperature condition.
However, the application of 100 mg L‑1 of chitosan resulted in an improvement in SPAD values whereas both dosages of chitosan led to an increase in total chlorophyll concentrations under stressful conditions.
Shoot length decreased under high temperatures and chitosan had no significant effect on shoot length in both stressed and non-stressed conditions.
Leaf relative water content significantly decreased in seedlings grown under heat stress and chitosan 100 mg L‑1 mitigated the adverse effects of it.
Overall, 100 mg L‑1 of chitosan can alleviate the adverse effect of high temperature stress in tomato seedlings.
Authors
M. Mozafarian, R. Najafi, E.P. Sari, Z. Pék, N. Kappel
Keywords
abiotic stress, chlorophyll, chitosan, heat stress
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