Articles
Effect of incubation temperature and gibberellic acid on dormancy breaking and seed germination of the protected plant Lilium martagon L.
Article number
1453_11
Pages
81 – 88
Language
English
Abstract
Lilium martagon L. is an attractive Greek native bulbous plant of Eurasiatic origin that is protected by the Greek Presidential Decree 67/1981. The aim of the present study was to investigate the effect of gibberellic acid treatment and incubation temperature on dormancy breaking and seed germination of L. martagon. The seeds were soaked in GA3 solutions of 500 and 1000 mg L‑1 for 24 h.
To determine the effect of temperature on seed germination, the GA3-treated seeds were randomly divided and placed in four temperature-controlled growth chambers (10, 15, 20 and 25°C) with a 12-h light/12-h dark photoperiod.
In addition, the seeds were soaked in distilled water for 24 h (control) and then placed in different temperature-controlled growth chambers to germinate.
After 10 weeks of incubation at 10, 15, 20 and 25°C, the control seeds only were subjected to cold stratification at 5°C for 12 weeks and then placed back at the former incubation temperatures.
The control seeds as well as the seeds treated with 500 and 1000 mg L‑1 exhibited the highest final germination percentage (29.00, 53.00 and 49.00%, respectively) at the incubation temperatures of 15°C. The treatment of seeds with GA3 significantly improved the germination at 10 and 15°C. At the incubation temperature of 25°C, seed germination was observed only after cold stratification of the control seeds.
However, in the germinated seeds which were planted in pots under greenhouse conditions, a significant delay was observed in the emergence of the aboveground part of the germinated seeds.
The knowledge of the germination requirements of L. martagon seeds will facilitate future conservation efforts as well as the sustainable exploitation of this species as a new native plant of ornamental value.
To determine the effect of temperature on seed germination, the GA3-treated seeds were randomly divided and placed in four temperature-controlled growth chambers (10, 15, 20 and 25°C) with a 12-h light/12-h dark photoperiod.
In addition, the seeds were soaked in distilled water for 24 h (control) and then placed in different temperature-controlled growth chambers to germinate.
After 10 weeks of incubation at 10, 15, 20 and 25°C, the control seeds only were subjected to cold stratification at 5°C for 12 weeks and then placed back at the former incubation temperatures.
The control seeds as well as the seeds treated with 500 and 1000 mg L‑1 exhibited the highest final germination percentage (29.00, 53.00 and 49.00%, respectively) at the incubation temperatures of 15°C. The treatment of seeds with GA3 significantly improved the germination at 10 and 15°C. At the incubation temperature of 25°C, seed germination was observed only after cold stratification of the control seeds.
However, in the germinated seeds which were planted in pots under greenhouse conditions, a significant delay was observed in the emergence of the aboveground part of the germinated seeds.
The knowledge of the germination requirements of L. martagon seeds will facilitate future conservation efforts as well as the sustainable exploitation of this species as a new native plant of ornamental value.
Authors
E. Pipinis, S. Hatzilazarou, I. Anestis, S. Panagiotopoulou, V. Anastasiadi, A. Stampoulidis, G. Tsoktouridis, N. Krigas, S. Kostas
Keywords
cold stratification, gibberellic acid, seed dormancy, sexual propagation
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