Articles
Comparative study of vegetative propagation methods of the medicinal plant Origanum scabrum
Article number
1453_27
Pages
195 – 202
Language
English
Abstract
Origanum scabrum Boiss. & Heldr. (Lamiaceae) is an endemic plant of Greece with aromatic and medicinal properties.
Plant propagation with botanical seeds is difficult and asexual propagation, by crown division or micropropagation, is an alternative approach to producing propagating material and preserving genetic material.
The differences in the development of plants from crown division derived from mother plants grown in pots and plants derived from micropropagation were studied.
Plants from micropropagation were produced from explants grown in modified semi-solid media MS with sucrose (30 g L‑1), agar (4 g L‑1) and pH = 5.8 and under T = 20°C, photoperiod = 16 h, light intensity = 40 μmol m‑2 s‑1. Microplants and plants from crown division were planted in trays with substrate peat and perlite (1:1, v:v), on April 26, 2024. After 24 days, plants were transplanted into 2-L pots with substrate peat and perlite (1:1, v/v). During the growth of the plants, the percentage (%) of growing plants, the number of shoots plant‑1, the number of leaves plant‑1, the height of the largest shoot on each plant and the number of flowering plants were calculated.
On the 120th day after transplantation, the fresh and dry weight of the above-ground part of the plant was measured and the dry matter content was calculated.
Both methods of propagation achieve a high plant survival rate (higher than 86%) without statistically significant differences.
Plants derived from micropropagation have higher growth rate, as shown by the rate of leaf and shoot emergence and by the height of the largest shoot of the plant.
In addition, plants derived from micropropagation have higher fresh and dry weight of the above-ground part of the plant, higher dry matter content (%). However, plants derived from micropropagation flowered 20 days later than plants derived from division.
Plant propagation with botanical seeds is difficult and asexual propagation, by crown division or micropropagation, is an alternative approach to producing propagating material and preserving genetic material.
The differences in the development of plants from crown division derived from mother plants grown in pots and plants derived from micropropagation were studied.
Plants from micropropagation were produced from explants grown in modified semi-solid media MS with sucrose (30 g L‑1), agar (4 g L‑1) and pH = 5.8 and under T = 20°C, photoperiod = 16 h, light intensity = 40 μmol m‑2 s‑1. Microplants and plants from crown division were planted in trays with substrate peat and perlite (1:1, v:v), on April 26, 2024. After 24 days, plants were transplanted into 2-L pots with substrate peat and perlite (1:1, v/v). During the growth of the plants, the percentage (%) of growing plants, the number of shoots plant‑1, the number of leaves plant‑1, the height of the largest shoot on each plant and the number of flowering plants were calculated.
On the 120th day after transplantation, the fresh and dry weight of the above-ground part of the plant was measured and the dry matter content was calculated.
Both methods of propagation achieve a high plant survival rate (higher than 86%) without statistically significant differences.
Plants derived from micropropagation have higher growth rate, as shown by the rate of leaf and shoot emergence and by the height of the largest shoot of the plant.
In addition, plants derived from micropropagation have higher fresh and dry weight of the above-ground part of the plant, higher dry matter content (%). However, plants derived from micropropagation flowered 20 days later than plants derived from division.
Authors
P. Fraskou, E. Kartsonas, C. Dordas, A.A. Alexopoulos
Keywords
aromatic and pharmaceutical plant, crown division, endemic plant, in vitro, Lamiaceae, micropropagation
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