Articles
Effect of different basal media on micropropagation of the medicinal plant Origanum scabrum (Boiss. & Heldr.)
Article number
1453_29
Pages
209 – 214
Language
English
Abstract
Micropropagation of the endemic medicinal plant Origanum scabrum Boiss. & Heldr. (Lamiaceae) is an effective method for producing propagating material and preserving plant genetic material.
The effect of the three different basal media, Murashige and Skoog (MS), woody plant medium (WPM), and Gamborgs’ medium (B5), as well as their concentrations (full strength, or half strength – ½), on the micropropagation of the O. scabrum was studied.
Node explants were transferred to Petri dishes with modified semi-solid media MS, ½ MS, WPM, ½ WPM, B5 and ½ B5. In all six treatments, sucrose (30 g L‑1) and agar (4 g L‑1) were added to the media, and the pH was adjusted to 5.8. In vitro cultures were transferred to growth chambers with T = 20°C, photoperiod = 16 h, light intensity = 40 μmol m‑2 s‑1. For each treatment 12 Petri dishes, with five explants each, were used.
Microplants cultivated in ½ MS basal media have high number of shoots, leaves, and nodes per explant, indicating the suitability of this basal media for rapid production of microplants that can be used as subcultures.
Microplants cultivated in the basal media ½ WPM had the slowest production rate of propagating units (nodes). On the other hand, microplants cultivated in the basal media with ½ WPM or with ½ B5 produced higher number of roots, indicating the suitability of these basal media to produce microplants for transplantation to the field.
After acclimatization for 14 days, microplants derived from all media showed high rates of survival during their growth in an unheated greenhouse for 68 days, where the plants derived from the ½ MS media and full-strength WPM produced a high number of leaves and lateral shoots.
The effect of the three different basal media, Murashige and Skoog (MS), woody plant medium (WPM), and Gamborgs’ medium (B5), as well as their concentrations (full strength, or half strength – ½), on the micropropagation of the O. scabrum was studied.
Node explants were transferred to Petri dishes with modified semi-solid media MS, ½ MS, WPM, ½ WPM, B5 and ½ B5. In all six treatments, sucrose (30 g L‑1) and agar (4 g L‑1) were added to the media, and the pH was adjusted to 5.8. In vitro cultures were transferred to growth chambers with T = 20°C, photoperiod = 16 h, light intensity = 40 μmol m‑2 s‑1. For each treatment 12 Petri dishes, with five explants each, were used.
Microplants cultivated in ½ MS basal media have high number of shoots, leaves, and nodes per explant, indicating the suitability of this basal media for rapid production of microplants that can be used as subcultures.
Microplants cultivated in the basal media ½ WPM had the slowest production rate of propagating units (nodes). On the other hand, microplants cultivated in the basal media with ½ WPM or with ½ B5 produced higher number of roots, indicating the suitability of these basal media to produce microplants for transplantation to the field.
After acclimatization for 14 days, microplants derived from all media showed high rates of survival during their growth in an unheated greenhouse for 68 days, where the plants derived from the ½ MS media and full-strength WPM produced a high number of leaves and lateral shoots.
Authors
E. Petroulaki, P. Fraskou, E. Mavrommati, A.A. Alexopoulos
Keywords
aromatic and medicinal plant, endemic plant, Gamborgs’ medium, in vitro, Lamiaceae, Murashige and Skoog, woody plant medium
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