Articles
IN VITRO EMBRYO CULTURE OF INTERSPECIFIC HYBRIDS BETWEEN THE KENYAN LINES OF PHASEOLUS VULGARIS L. AND PHASEOLUS ACUTIFOLIUS A. GRAY
Article number
218_27
Pages
221 – 230
Language
Abstract
The objective of the study was to examine interspecific compatibility between the Kenyan Phaseolus vulgaris lines (GLP 2, GLP 24, GLP 1004, GLP x 92 and 1122) and Phaseolus acutifolius lines (P.I. 310801, P.I. 310606, P.I. 263590, L-246–2, L-179, CIAT G40016, Buff Hood Arizona and White Hood Arizona) by in vitro embryo culture, with regard to future genetic exchange between the two species.
Successful hybrids were procured between GLP24 x Buff Hood Arizona, GLP 1004 x CIAT G40016, 1122 x P.I. 310801.
Rooting, which was poor among the interspecific hybrids produced in vitro, was promoted either by altering media composition or trying various genotype combination.
N°-benzyladenine promoted shoot development and retarded rooting of hybrids in vitro.
Exclusion of hormones promoted both shoot and root development.
Publication
Authors
S.D. Obukosia, K. Waithaka, V.K. Gupta, P.M. Kimani
Keywords
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