Articles
PRESUMPTIVE TRANSFORMATION OF APPLES BY CO-CULTIVATION WITH AGROBACTERIUM TUMEFACIENS
Article number
240_9
Pages
59 – 62
Language
Abstract
Presumptive transformed callus and shoots were produced by a leaf disc transformation-regeneration method.
Leaf explants excised from in vitro grown shoot cultures of ‘Red Delicious’ apple (Malus x domestica Borkh.) were co-cultivated with Agrobacterium tumefaciens strain containing either the virulent plasmid C58 or the disarmed plasmid pGV3850::1103neo.
After co-cultivation for 36 hours, the leaf pieces were placed on the appropriate selection medium and incubated in the dark at 26°C. On average 10% of the leaf pieces were able to form calli under selection conditions for phytohormone-independent growth, in contrast to controls which did not form calli.
Abnormal shoots were formed from about 2% of the C58 transformed calli, while normal shoots were formed from about 1% of the pGV3850::1103neo.
The presumptive presence of nopaline-synthase activity in the calli provided evidence of expression of T DNA genes and genetic transformation.
Leaf explants excised from in vitro grown shoot cultures of ‘Red Delicious’ apple (Malus x domestica Borkh.) were co-cultivated with Agrobacterium tumefaciens strain containing either the virulent plasmid C58 or the disarmed plasmid pGV3850::1103neo.
After co-cultivation for 36 hours, the leaf pieces were placed on the appropriate selection medium and incubated in the dark at 26°C. On average 10% of the leaf pieces were able to form calli under selection conditions for phytohormone-independent growth, in contrast to controls which did not form calli.
Abnormal shoots were formed from about 2% of the C58 transformed calli, while normal shoots were formed from about 1% of the pGV3850::1103neo.
The presumptive presence of nopaline-synthase activity in the calli provided evidence of expression of T DNA genes and genetic transformation.
Publication
Authors
P.A. Mooney, P.B. Goodwin
Keywords
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