Articles
REGENERATION OF CARNATION (DIANTHUS CARYOPHYLLUS) USING LEAF EXPLANTS
Article number
307_12
Pages
109 – 116
Language
Abstract
A regeneration procedure was developed for carnation (Dianthus caryophyllus L.) using leaves from in vitro grown plants.
Leaves were carefully removed from the stem using forceps.
Basal explants were incubated on MS medium with 0.3 mg/l BA and 0.3 mg/l NAA. Adventitious shoots developed after two weeks at the bases of the explants.
Growth and development of regenerants was normal without aberrations like vitrification or premature flowering, often observed in regeneration of carnation.
The regeneration procedure was generally applicable as shown by the regeneration of 24 out of 25 cultivars from various carnation types.
To further optimize the procedure, a number of factors was analyzed.
The age of leaves appeared to be important.
The youngest leaves close to the apical meristem gave the best regeneration results, but the age of the plants did not have much effect on regeneration.
Effects of cytokinin BA and auxin NAA on regeneration were investigated using concentrations of 0, 0.1, 0.3, 0.9 and 2.7 mg/l for both BA and NAA. At concentrations of BA and NAA from 0.1 to 0.9 mg/l, the regeneration frequency expressed as the percen-tage explants showing regeneration, was optimal (23 %). The highest numbers of adventitious shoots per explant were obtained at 0.9 mg/l BA and 0.3 mg/l NAA.
Leaves were carefully removed from the stem using forceps.
Basal explants were incubated on MS medium with 0.3 mg/l BA and 0.3 mg/l NAA. Adventitious shoots developed after two weeks at the bases of the explants.
Growth and development of regenerants was normal without aberrations like vitrification or premature flowering, often observed in regeneration of carnation.
The regeneration procedure was generally applicable as shown by the regeneration of 24 out of 25 cultivars from various carnation types.
To further optimize the procedure, a number of factors was analyzed.
The age of leaves appeared to be important.
The youngest leaves close to the apical meristem gave the best regeneration results, but the age of the plants did not have much effect on regeneration.
Effects of cytokinin BA and auxin NAA on regeneration were investigated using concentrations of 0, 0.1, 0.3, 0.9 and 2.7 mg/l for both BA and NAA. At concentrations of BA and NAA from 0.1 to 0.9 mg/l, the regeneration frequency expressed as the percen-tage explants showing regeneration, was optimal (23 %). The highest numbers of adventitious shoots per explant were obtained at 0.9 mg/l BA and 0.3 mg/l NAA.
Publication
Authors
A.C. van Altvorst, T. Bruinsma, H.J.J. Koehorst, J.J.M. Dons
Keywords
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