Articles
INFLUENCE OF TEMPERATURE ON THE ULTRASTRUCTURE AND SEROLOGICAL REACTIVITY OF TWO TOSPOVIRUS ISOLATES
Article number
377_15
Pages
149 – 158
Language
Abstract
Nicotiana benthamiana plants infected with a gloxinia isolate of Impatiens necrotic spot virus INSV (INSV-Igg) were grown in a greenhouse with an average temperature of 32 C. Clusters of virions appeared in infected plants and there was a reduction in the amount of chain-like N protein after three serial passages.
In two controlled experiments at 27/24 C, light/dark (high temperature), many virions were produced by the third serial passage.
By the tenth passage fewer chain-like aggregates of N protein were observed.
In one experiment, chain-like aggregates of N protein were absent in plants grown at 27/24 C. ELISA tests with INSV-I antiserum and immunogold labelling with the same antibody were negative for INSV from the third through the tenth passage.
This modified high temperature culture remained stable after repeated high temperature as well as low temperature (21/18 C) passage in tobacco.
The cytopathology of infected N. benthamiana plants grown at 21/18 C, light/dark and inoculated with the original INSV-Igg culture remained as the original, with few or no virions present.
Since cytopathology and serological reactivity of INSV-Igg may change with temperature, these properties should not be considered stable criteria in classification of the virus.
In two controlled experiments at 27/24 C, light/dark (high temperature), many virions were produced by the third serial passage.
By the tenth passage fewer chain-like aggregates of N protein were observed.
In one experiment, chain-like aggregates of N protein were absent in plants grown at 27/24 C. ELISA tests with INSV-I antiserum and immunogold labelling with the same antibody were negative for INSV from the third through the tenth passage.
This modified high temperature culture remained stable after repeated high temperature as well as low temperature (21/18 C) passage in tobacco.
The cytopathology of infected N. benthamiana plants grown at 21/18 C, light/dark and inoculated with the original INSV-Igg culture remained as the original, with few or no virions present.
Since cytopathology and serological reactivity of INSV-Igg may change with temperature, these properties should not be considered stable criteria in classification of the virus.
Authors
R.H. Lawson, M.M. Dienelt, H.T. Hsu
Keywords
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