Articles
CHARACTERIZATION OF AN ILARVIRUS FROM LISIANTHUS (EUSTOMA GRANDIFLORUM)
Article number
377_7
Pages
81 – 90
Language
Abstract
A virus with quasi-isometric particles was isolated from lisianthus (Eustoma grandiflorum, syn. Lisianthus russellianum) showing poor growth and necrotic line patterns on the leaves.
The virus was sap-transmissible and had a wide experimental host range.
The disease was experimentally reproduced on healthy lisianthus seedlings.
The virus was purified and sera were prepared by injecting rabbits with virus preparations fixed with either glutaraldehyde or dithiobis (succinimidyl propionate). Solubilized virus particles yielded one coat protein of Mr 24.7 kDa as estimated by PAGE. Total RNA extracted from particles was infective and formed 5 bands with estimated Mrs of 1.03, 0.85, 0.64, 0.30 and 0.25 MDa after electrophoresis in 1% denaturing agarose gels.
Serological relationships were determined by decoration immunoelectron microscopy, indirect plate-trapping ELISA and Western blotting.
The virus was serologically closely related, but not identical to parietaria mottle (ParMV) and more distantly to tobacco streak virus, but not to seven other ilarviruses.
The host range differed from that of ParMV. We therefore suggest that the lisianthus virus is distinct and propose the name lisianthus line pattern virus.
The virus was sap-transmissible and had a wide experimental host range.
The disease was experimentally reproduced on healthy lisianthus seedlings.
The virus was purified and sera were prepared by injecting rabbits with virus preparations fixed with either glutaraldehyde or dithiobis (succinimidyl propionate). Solubilized virus particles yielded one coat protein of Mr 24.7 kDa as estimated by PAGE. Total RNA extracted from particles was infective and formed 5 bands with estimated Mrs of 1.03, 0.85, 0.64, 0.30 and 0.25 MDa after electrophoresis in 1% denaturing agarose gels.
Serological relationships were determined by decoration immunoelectron microscopy, indirect plate-trapping ELISA and Western blotting.
The virus was serologically closely related, but not identical to parietaria mottle (ParMV) and more distantly to tobacco streak virus, but not to seven other ilarviruses.
The host range differed from that of ParMV. We therefore suggest that the lisianthus virus is distinct and propose the name lisianthus line pattern virus.
Authors
V. Lisa, A.M. Vaira, M. d’Aquilio, G. Dellavalle, V. Masenga, R.G. Milne, G. Boccardo
Keywords
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