Articles
ELECTRON MICROSCOPICAL AND SEROLOGICAL STUDIES ON FOUR ISOMETRICAL PELARGONIUM VIRUSES
The cytological effects of PLCV are long known and mainly characterized by induction of peroxisome membrane-associated vesicles, which were not induced by the three carmoviruses studied.
PFBV induced very characteristic dilations of mitochondrial cristae which contained fibrillar material.
The fibrillae were dissimilar to dsDNA in mitochondria and chloroplasts and to dsRNA of tombusvirus-induced membrane-associated vesicles.
Alterations induced by PLPV and PeRSV were similar to each other and comprised mainly accumulations of granular dark staining material as cytoplasmic inclusions which appeared to be composed of spherical elements larger than virions.
Cytoplasmic accumulations of lipid droplets were also induced by both viruses.
The four viruses were clearly differentiated in decoration tests and showed no serological relations.
PLPV was not decorated by antisera to 35 tombus- and carmoviruses except the homologous antiserum.
PeRSV showed the homologous reaction and the already known reaction with antiserum to elderberry latent carmovirus but did not react with the other antisera tested.
Five isolates of PFBV revealed no serological differentiation when tested with four antisera in DAS-ELISA. Likewise two isolates of PLPV were not differentiated from each other using two antisera in DAS-ELISA.