Articles
EVALUATION OF FINGERPRINTING TECHNIQUES FOR DIFFERENTIATION OF CRANBERRY AND BLUEBERRY VARIETIES.
Article number
446_35
Pages
239 – 242
Language
Abstract
Most varieties of cranberry commonly grown today are from native selections made in the 1800s or cultivars introduced in the mid 1900s.
The lack of distinct morphological traits, coupled with factors such as contamination of cultivated bogs with off-type varieties, has led to a wide mixture of individuals grown under a single cultivar name.
Although misidentification of blueberries is likely to be less common, a rapid method of cultivar identification for both crops would be very useful.
Two fingerprinting techniques were evaluated for this purpose, RAPD (random amplified polymorphic DNA) analysis and AFLP (amplified fragment length polymorphism) analysis.
Both methods are PCR based and thus allow the use of small amounts of DNA. RAPDs have been shown to be useful in identification of cranberries, but the method is prone to artifacts and requires several reactions and primers for analysis of one individual.
More steps are required for the AFLP procedure and the cost is consequently higher per reaction as compared to RAPDs.
The units of information (i.e. number of polymorphic bands) from a single AFLP reaction however, are greater than those from a typical RAPD reaction.
This reduces the relative cost of AFLPs and increases speed and accuracy of identification.
The lack of distinct morphological traits, coupled with factors such as contamination of cultivated bogs with off-type varieties, has led to a wide mixture of individuals grown under a single cultivar name.
Although misidentification of blueberries is likely to be less common, a rapid method of cultivar identification for both crops would be very useful.
Two fingerprinting techniques were evaluated for this purpose, RAPD (random amplified polymorphic DNA) analysis and AFLP (amplified fragment length polymorphism) analysis.
Both methods are PCR based and thus allow the use of small amounts of DNA. RAPDs have been shown to be useful in identification of cranberries, but the method is prone to artifacts and requires several reactions and primers for analysis of one individual.
More steps are required for the AFLP procedure and the cost is consequently higher per reaction as compared to RAPDs.
The units of information (i.e. number of polymorphic bands) from a single AFLP reaction however, are greater than those from a typical RAPD reaction.
This reduces the relative cost of AFLPs and increases speed and accuracy of identification.
Publication
Authors
James J. Polashock, N. Vorsa
Keywords
RAPD, AFLP, genotyping
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