Home > International Symposium on Molecular Markers for Characterizing Genotypes and Identifying Cultivars in Horticulture > RAPD AND AFLP GENETIC MARKERS FOR THE CHARACTERISATION OF OSTEOSPERMUM GERMPLASM

Articles

RAPD AND AFLP GENETIC MARKERS FOR THE CHARACTERISATION OF OSTEOSPERMUM GERMPLASM

Article number

546_17

Pages

171 – 176

Language

English

Abstract

The level of genetic diversity was assayed within the accessions of three breeding groups in an Osteospermum collection.
The DNA-profiling via RAPD revealed high levels of polymorphism.
Genetic variation was highest in the English material, intermediate in the Italian and lowest in the Danish breeding group.
The most frequent similarity classes were 0.5-0.6 for the English, 0.6-0.7 for the Italian and 0.7-0.8 for the Danish group.
Furthermore, an AFLP analysis has been carried out on a sample of eight accessions representative of the three groups.
Preliminary results indicate that AFLP analysis is suitable to give polymorphism in Osteospermum. RAPD and AFLP analysis can therefore be a useful tool for evaluating genetic diversity for breeding purposes, and for protecting intellectual property rights of new varieties.

Publication

International Symposium on Molecular Markers for Characterizing Genotypes and Identifying Cultivars in Horticulture

Authors

T. Berio, G. Morreale, A. Giovannini, L. Allavena, L. Arru, P. Faccioli, V. Terzi, N. Pecchioni

Keywords

African Daisy, molecular markers, genetic similarity, biodiversity

Full text

https://doi.org/10.17660/ActaHortic.2001.546.17

Online Articles (90)

1. CONCEPTUAL AND STATISTICAL ISSUES RELATED TO THE USE OF MOLECULAR MARKERS FOR DISTINCTNESS AND ESSENTIAL DERIVATION

F.A. van Eeuwijk | C.P. Baril

2. GENETIC DISTANCE ESTIMATORS BASED ON MOLECULAR DATA FOR PLANT REGISTRATION AND PROTECTION: A REVIEW

V. Lombard | P. Dubreuil | C. Dilmann | C. Baril

3. VARIETAL DISTINCTNESS ASSISTED BY MOLECULAR MARKERS: A METHODOLOGICAL APPROACH

G. Nuel | C. Baril | S. Robin

4. MATHEMATICAL ANALYSIS OF RAPD DATA TO ESTABLISH RELIABILITY OF VARIETAL ASSIGNMENT IN VEGETATIVELY PROPAGATED SPECIES

J. Ibañez

5. AN OPERATIONAL BAYESIAN APPROACH FOR THE IDENTIFICATION OF SEXUALLY REPRODUCED CROSS FERTILIZED POPULATIONS USING MOLECULAR MARKERS

L. Baudouin | P. Lebrun

7. COMPARISON OF MORPHOLOGICAL CHARACTERISTICS AND RAPD FOR ESTIMATING GENETIC DIVERSITY IN ABELMOSCHUS SPP.

G.E. Martinello | N.R. Leal | A.T. Amaral Jr. | M.G. Pereira | R.F. Daher

8. MOLECULAR METHODS IN THE CONSERVATION AND USE OF PLANT GENETIC RESOURCES

T. Hodgkin | R. Roviglioni | M.C. De Vicente | N. Dudnik

9. CHARACTERISATION OF VINEYARD PEACH BIODIVERSITY

K. Gašic | V. Ognjanov | R. Boškovi´c | K.R. Tobutt | C. James

10. EVALUATION OF CARROT GENETIC SUBSTRUCTURE: COMPARISON OF THE EFFICIENCY OF MAPPED MOLECULAR MARKERS WITH RANDOMLY CHOSEN MARKERS

V. Le Clerc | M. Briard | D. Peltier

11. GENETIC DIVERSITY IN RIBES

P.G. Lanham | R.M. Brennan

12. BETWEEN- AND WITHIN POPULATION DIVERSITY IN DOGROSE SPECIES

H. Nybom | G. Werlemark | A.M.E. Olsson

13. DNA MICROSATELLITE IN FRUIT CROPS: ISOLATION, LENGTH POLYMORPHISM, INHERITANCE, SOMATIC STABILITY AND CROSS-SPECIES CONSERVATION

G. Cipriani | M.T. Marrazzo | G. Di Gaspero | R. Testolin

14. USE OF AFLP MARKER TECHNIQUE FOR CLASSIFICATION OF ALSTROEMERIA SPP. AND CONSTRUCTION OF A LINKAGE MAP IN ALSTROEMERIA AUREA

T.H. Han | H.J. van Eck | M.J. de Jeu | E. Jacobsen

15. MOLECULAR MARKERS IN ALLIUM: RANGE OF APPLICATION, RELIABILITY AND TAXONOMIC IMPLICATIONS

16. EVALUATION OF GENETIC DIVERSITY IN A GARDEN BEET GERMPLASM COLLECTION USING RAPD TECHNIQUE

R. Baranski | D. Grzebelus | L. Frese

17. RAPD AND AFLP GENETIC MARKERS FOR THE CHARACTERISATION OF OSTEOSPERMUM GERMPLASM

18. ASSESSMENT OF THE GENETIC RELATIONSHIPS AMONG CITRUS SPECIES AND VARIETIES BY ISOZYME AND RAPD MARKERS

L. Cabrita | P. Elisiário | J. Leitão | A. Guerreiro

19. THE USE OF AFLP MARKERS FOR CHARACTERIZATION OF BIODIVERSITY IN INDIGENOUS TREES AND DATA ANALYSIS

E. Coart | V. Lamote | J. De Riek | E. Van Bockstaele | M. De Loose

20. ESTIMATION OF HETEROZYGOSITY IN TWO BOTANIC ROSE SPECIES USING AFLP MARKERS

L. Crespel | D. Zhang | J. Meynet | S. Gudin

21. DEVELOPMENT OF SEQUENCED TAGGED MICROSATELLITE SITE (STMS) MARKERS IN AZALEA

22. CHARACTERIZATION OF MICROSATELLITE FLANKING SEQUENCES IN VITIS SPECIES

G. Di Gaspero | E. Peterlunger | R. Testolin | G. Cipriani

23. GENETIC RELATIONSHIPS BETWEEN APRICOT (Prunus armeniaca L.) AND RELATED SPECIES USING AFLP MARKERS

L.S. Hagen | P. Lambert | J.M. Audergon | B. Khadari

24. IDENTIFICATION OF APRICOT (Prunus armeniaca L.) GENOTYPES USING MICROSATELLITE AND RAPD MARKERS

J.I. Hormaza

25. CHARACTERIZATION OF ACER GRISEUM AND ITS PUTATIVE INTERSPECIFIC HYBRIDS

Y.H. Joung | M.S. Roh | S.E. Bentz

26. THE USE OF MICROSATELLITE MARKERS TO EXAMINE THE DIVERSITY OF THE GENETIC RESOURCES OF RICE (Oryza sativa) ADAPTED TO EUROPEAN CONDITIONS

C. Luce | J.L. Noyer | D. Tharreau | N. Ahmadi | H. Feyt

27. EVALUATION OF GENETIC RELATIONSHIPS IN CITRUS GENUS BY MEANS OF SEQUENCE TAGGED MICROSATELLITES

F. Luro | D. Rist | P. Ollitrault

28. DNA FINGERPRINTING OF SWEETPOTATO (Ipomoea batatas L.): TWO CASE STUDIES IN AFRICA

C. McGregor | M. Greyling | J. Banda | S. Laurie

29. GENETIC DIVERSITY OF A FERAL POPULATION OF OLIVE TREES SURROUNDING AN ABANDONED GROVE

G. Mekuria | G. Collins | M. Sedgley

30. GENETIC VARIATION IN WILD AND CULTIVATED MATERIAL OF BLACK CHOKEBERRY

H.A. Persson | N. Jeppsson | H. Nybom

31. S-HAPLOTYPE POLYMORPHISM IN BRASSICA OLERACEA

V. Ruffio-Chable | T. Gaude

32. STRUCTURATION OF GENETIC DIVERSITY IN CULTIVATED AND WILD CHERRY TREES USING AFLP MARKERS

M. Tavaud | A. Zanetto | F. Santi | E. Dirlewanger

33. CHARACTERISATION OF GRAPEVINE ACCESSIONS AT GERMPLASM BANKS WITH RAPD AND MICROSATELLITE MARKERS

S. Ulanowsky | Y. Gogorcena | F. Martinez-de-Toda | J. Borrego | J. Ibanez

34. MOLECULAR MARKERS IN DETECTION OF DISTANT HYBRIDS IN LILIUM

K. Wiejacha | A. Marasek | I. Sabala | T. Orlikowska

35. POSSIBLE FUTURE ROLES FOR MOLECULAR TECHNIQUES IN THE IDENTIFICATION AND REGISTRATION OF NEW PLANT CULTIVARS

M.S. Camlin

36. COMPARISON OF FRUIT AND LEAF DNA EXTRACTS FOR AFLP AND SSR ANALYSIS IN PEACH (Prunus persica (L.) Batsch)

M.J. Aranzana | M.C. de Vicente | P. Arús

37. ARE AFLP MARKERS THE BEST ALTERNATIVE FOR CULTIVAR IDENTIFICATION?

G. Arnau | J. Lallemand | M. Bourgoin

38. STANDARDIZATION AND APPLICATION OF MICROSATELLITE MARKERS FOR VARIETY IDENTIFICATION IN TOMATO AND WHEAT

39. OLIVE CULTIVAR IDENTIFICATION USING NUCLEAR RAPDS AND MITOCHONDRIAL RFLPS

G. Besnard | P. Baradat | A. Bervillé

40. DNA FINGERPRINTS FOR PELARGONIUM CULTIVAR IDENTIFICATION

41. MOLECULAR MARKERS FOR GENOTYPING GRAPEVINE AND FOR IDENTIFYING CLONES OF TRADITIONAL VARIETIES

F. Regner | A. Stadlbauer | C. Eisenheld

42. INTRAVARIETAL DNA POLYMORPHISMS IN GRAPEVINE (Vitis vinifera L.)

D. Bellin | R. Velasco | M.S. Grando

43. EVALUATION OF AFLPS FOR VARIETY IDENTIFICATION IN MODERN ROSE (Rosa hybrida L.)

D. Zhang | C. Besse | M.Q. Cao | M.H. Gandelin

44. GENETIC VARIATION OF THE SELF-INCOMPATIBILITY ALLELES (S-ALLELES) IN THE CULTIVATED RADISH (Raphanus sativus L.) BY THE PCR-RFLP METHOD

S. Niikura | S. Matsuura

45. AFLP AND SSR MARKERS FOR GENETIC DIVERSITY ANALYSIS AND CULTIVAR IDENTIFICATION IN PEACH [Prunus persica (L.) Batsch]

M.J. Aranzana | P. Arús | J. Carbó | G.J. King

46. CHARACTERISATION OF THE MOST IMPORTANT SPANISH GRAPE VARIETIES THROUGH ISOZYME AND MICROSATELLITE ANALYSIS

47. A COMPARATIVE STUDY ON THE USE OF ISSR, MICROSATELLITES AND RAPD MARKERS FOR VARIETAL IDENTIFICATION OF CARROT GENOTYPES

M. Briard | V. Le Clerc | A.E. Mausset | A. Veret

48. IDENTIFICATION OF POTATO VARIETIES BY DNA PROFILING

G. Corbett | D. Lee | P. Donini | R.J. Cooke

49. GENOTYPE IDENTIFICATION OF ORNAMENTAL SPECIES BY RAPD ANALYSIS

L. De Benedetti | A. Mercuri | S. Bruna | G. Burchi | T. Schiva

50. GENETIC CHARACTERISATION OF ITALIAN WINE GRAPE CULTIVARS BY MICROSATELLITE DNA ANALYSIS

I. Filippetti | O. Silvestroni | M.R. Thomas | C. Intrieri

51. APPLICATION OF MICROSATELLITE-BASED DNA PROFILING IN ACTINIDIA SPECIES

L.G. Fraser | C.F. Harvey | G.P. Gill

52. AN INVESTIGATION INTO THE IDENTITY OF THE PERENNIAL CULTIVARS OF LAVATERA L. USING MORPHOLOGY AND RAPD MARKERS

M.L. Grant | D.M. Miller | A. Culham

53. COMPARISON OF RAPD AND AFLP TECHNIQUES USED FOR THE EVALUATION OF GENETIC DIVERSITY OF CARROT BREEDING MATERIALS

D. Grzebelus | R. Baranski | B. Jagosz | B. Michalik | P.W. Simon

54. CONTRIBUTION TO APRICOT GENETIC ANALYSIS WITH RFLP, RAPD AND AFLP MARKERS

55. FORCING AND MOLECULAR CHARACTERIZATION OF CAMPANULA

Y.H. Joung | M.S. Roh | T.I. Kim | J.S. Song

56. THE USE OF MICROSATELLITES TO ESTABLISH UNIQUE FINGERPRINTS FOR APPLE CULTIVARS AND SOME OF THEIR DESCENDANTS

K. Kenis | E. Pauwels | N. Van Houtvinck | J. Keulemans

57. MOLECULAR CHARACTERIZATION AND GENETIC STRUCTURE OF OLIVE GERMPLASM COLLECTION IN CONSERVATOIRE BOTANIQUE NATIONAL MÉDITERRANÉEN DE PORQUEROLLES USING NUCLEAR RAPD MARKERS AND RFLP OF MITOCHONDRIAL DNA

B. Khadari | C. Breton | G. Besnard | J.P. Roger | A. Bervillé

58. GENETIC DIVERSITY OF MOROCCAN CULTIVATED OLIVE USING RAPD MARKERS

B. Khadari | A. Bervillé

59. RANDOMLY AMPLIFIED POLYMORPHIC DNA (RAPD) VARIATION AMONG AND WITHIN POPULATIONS OF ARTICHOKE, CULTIVAR ”SPINOSO SARDO

S. Lanteri | I. Di Leo | E. Portis | L. Quagliotti

60. MOLECULAR ANALYSIS OF THE GENETIC VARIABILITY OF PORTUGUESE ALMOND COLLECTIONS

61. TOMATO ECOTYPE CHARACTERIZATION BY ANIONIC EXCHANGE¿HIGH PERFORMANCE LIQUID CHROMATOGRAPHY ANALYSIS OF ENDOSPERM SEED PROTEINS

G. Mennella | V. Onofaro Sanajà | A. D'Alessandro

62. ASSESSING COMMON BEAN GENETIC DIVERSITY USING RFLP, DAMD-PCR, ISSR, RAPD AND AFLP MARKERS

I. Métais | C. Aubry | B. Hamon | R. Jalouzot | D. Peltier

63. CHLOROPLAST DNA DIVERSITY AND DISTINGUISHING SWEET CHERRY CULTIVARS: A NEW APPROACH

A. Mohanty | J.P. Martín | I. Aguinagalde

64. POTATO CULTIVARS IDENTIFICATION USING MOLECULAR MARKERS

M. Moisan-Thiéry | Y. Le Hingrat | M.C. Kerlan

65. MOLECULAR EVIDENCE FOR THE ORIGIN OF ACHILLEA ‘TAYGETEA’ OF GARDENS

C. Pankhurst | J. Compton | S. Thornton-Wood | J. Cubey | A. Culham

66. CHARACTERIZATION OF TOMATO GENOTYPES THAT DIFFER IN THEIR FRUIT SHELFLIFE BY ANALYSIS OF TOTAL PERICARP PROTEIN PATTERNS AT TWO RIPENING STAGES

G.R. Pratta | R. Zorzoli | L.A. Picardi | E.M. Valle | N. Carrillo

67. CHARACTERIZATION AND EVALUATION OF GENETIC DIVERSITY IN PEACH GERMPLASM USING RAPD AND RFLP MARKERS

R. Quarta | M.T. Dettori | I. Verde | U. Marchesi | M.A. Palombi

68. GENETIC DIVERSITY IN COWPEA (Vigna unguiculata) USING ISOZYME ELECTROPHORESIS

C.M. Reis | A.M. Frederico

69. MOLECULAR MARKERS AND GENETIC BASE BROADENING IN LYCOPERSICON SPP.

G. Saavedra | W. Spoor | L. Harrier

70. DISTINCTION BETWEEN CLOSELYRELATED APPLE CULTIVARS OF THE BELLE-FLEUR FAMILY USING RFLP AND AFLP MARKERS

M. Tignon | M. Lateur | R. Kettmann | B. Watillon

71. IDENTIFICATION OF COPIA-LIKE RETROTRANSPOSABLE ELEMENT BY APPLE

M. Tignon | B. Watillon | R. Kettmann

72. IDENTIFICATION OF CICHORIUM INTYBUS L. AND THEIR PHENETIC RELATIONSHIP REVEALED BY RAPDS

N. Van Stallen | V. Noten | M. Demeulemeester | M.P. De Proft

73. ROUTINE EXTRACTION OF DNA FROM GRAPEVINE (Vitis ssp.) CANES AND ROOTS FOR VARIETY IDENTIFICATION BY RAPD-PCR

T. Wolf | C. Ortlieb | K. Eimert | R. Ries

74. IDENTIFYING DUPLICATES IN SWEET POTATO [Ipomoea batatas (L.) Lam] CULTIVARS USING RAPD

D.P. Zhang | Z. Huaman | F. Rodriguez | G. Rossel | M. Ghislain

75. IDENTIFICATION OF CHINESE CABBAGE (Brassica campestris L. ssp. Pekinensis) CULTIVARS WITH ISOZYME, RAPD AND AFLP MARKERS

X. Zheng | S. Song | H. Liu

76. IDENTIFICATION OF GENOTYPES USING AFLP MARKERS IN APPLE ROOTSTOCKS

J. Zhu | A.Q. Zhou | H.Y. Dai | G.C. Li

77. MARKER-ASSISTED SELECTION FOR THE TRANSFER OF QTLS CONTROLLING FRUIT QUALITY TRAITS INTO TOMATO ELITE LINES

M. Causse | L. Lecomte | N. Baffert | P. Duffé | F. Hospital

78. IDENTIFICATION OF A RAPD MARKER LINKED TO TOLERANCE TO PEACOCK DISEASE (Spilocea oleaginea) IN OLIVES (Olea europaea)

G. Mekuria | G. Collins | S. Lavee | M. Sedgley

79. TRANSGENE-BASED RESISTANCE TO PLUM POX VIRUS (SHARKA DISEASE) IS TRANSFERRED THROUGH INTERSPECIFIC HYBRIDIZATION IN PRUNUS

M. Ravelonandro | P. Briard | R. Renaud | R. Scorza

80. MOLECULAR IDENTIFICATION OF S-GENOTYPES OF ALMOND (Prunus dulcis)

Ma | M.M. Oliveira

81. MARKER ASSISTED SELECTION FOR SCAB RESISTANT APPLE

L. Giongo | A. Bergamini | R. Rigatti | M. Komjanc

82. AFLP MARKERS FOR SEX DETERMINATION IN AN ILEX SPECIES

A. Golan-Goldhirsch | R. Jones | L.J. Rowland

83. DEVELOPMENT OF DNA MARKERS LINKED TO BACTERIAL LEAF SPOT RESISTANCE OF CHILLI

K.T. Kim | H.S. Choi | H.J. Kim | D.H. Pae | J.Y. Yoon

84. DETECTION OF BEAN SEED-BORNE PATHOGENS BY PCR

F. Molouba | C. Guimier | C. Berthier

85. USE OF DIFFERENT MOLECULAR MARKERS, RAPDS AND SSRS, TO INVESTIGATE CLONAL STABILITY IN MICROPROPAGATED KIWIFRUIT (Actinidia deliciosa A. Chev.)

M.A. Palombi | C. Damiano

86. IDENTIFICATION OF MOLECULAR MARKERS LINKED TO POWDERY MILDEW (Erysiphe pisi Syd.) RESISTANCE MUTATED GENES IN PISUM SATIVUM L.

G. Pereira | M.T. Sousa | J.M. Leitão

87. AFLP MARKER DEVELOPMENT IN ROSE FOR GENETIC MAPPING: COMPARISON OF THREE RESTRICTION ENZYME PAIRS

S. Rajapakse | L. Zhang | R.E. Ballard | D.H. Byrne

88. CHALLENGES IN DEVELOPING MOLECULAR MARKERS FOR ALMOND (Prunus amygdalus) AND GRAPE (Vitis species)

89. GENETIC ANALYSIS OF A POLISH APPLE SELECTION U 211 AS A NEW SOURCE OF HIGH RESISTANCE TO APPLE POWDERY MILDEW

M. Stankiewicz | E. Pitera | S.W. Gawronski

90. INTROGRESSION OF A PARTIAL RESISTANCE TO PHYTOPHTHORA CAPSICI LEON. INTO A PEPPER ELITE LINE BY MARKER ASSISTED BACKCROSSES