GENETIC VARIATION OF THE SELF-INCOMPATIBILITY ALLELES (S-ALLELES) IN THE CULTIVATED RADISH (Raphanus sativus L.) BY THE PCR-RFLP METHOD

Radish exhibits sporophytic self-incompatibility with multiple S-alleles located at a single locus.
Most radish cultivars are becoming F₁ hybrids and the hybrid seeds are produced using self-incompatibility.
Thus, it is very significant that the S-genotypes of the plants can be identified before flowering using the PCR-RFLP method.
We aimed to identify the S-allele genetic variations of the cultivated radish and distinguish usable S-alleles for F₁ seed production.
We investigated 71 inbred lines, which are homozygous at the S-locus and used as parents of commercial F₁ hybrids, and 219 lines belonging to 23 local varietal groups; 18 Japanese varietal groups and 5 non-Japanese varietal groups (from Europe, Korea, Nepal, North China and South China). Thirty-seven S-alleles, designated as S²⁰¹ to S²³⁷, were identified.
Twenty-nine of the thirty-seven S-alleles could be amplified by the PCR and identified by MspI-digestion.
No PCR products were found in the other S-alleles.
We will discuss the diversification of the S-alleles in the radish.

International Symposium on Molecular Markers for Characterizing Genotypes and Identifying Cultivars in Horticulture

S. Niikura, S. Matsuura

F1 hybrid, seed production, S-genotype, S-locus, SLG

https://doi.org/10.17660/ActaHortic.2001.546.44