Home > International Symposium on Molecular Markers for Characterizing Genotypes and Identifying Cultivars in Horticulture > TRANSGENE-BASED RESISTANCE TO PLUM POX VIRUS (SHARKA DISEASE) IS TRANSFERRED THROUGH INTERSPECIFIC HYBRIDIZATION IN PRUNUS

Articles

TRANSGENE-BASED RESISTANCE TO PLUM POX VIRUS (SHARKA DISEASE) IS TRANSFERRED THROUGH INTERSPECIFIC HYBRIDIZATION IN PRUNUS

Article number

546_79

Pages

569 – 574

Language

Abstract

Plum pox virus (PPV) resistant rootstocks would be useful to control the spread of PPV in nurseries and orchards.
The PPV-resistant transgenic Prunus domestica plum clone C-5 was used as a parent with Prunus spinosa to develop PPV resistant hybrid progeny.
This work showed that C-5 plum is sexually compatible with the Prunus rootstock species P. spinosa. Resistance assays of chip-bud inoculated plants following two cycles of growth and cold-induced dormancy demonstrated that the transgenic hybrid clones were symptomless and DAS-ELISA negative.
Further, immuno-blotting failed to detect PPV CP in inoculated transgenic hybrids.
These results demonstrated the usefulness of C-5 for developing PPV resistant rootstocks with P. spinosa.

Publication

International Symposium on Molecular Markers for Characterizing Genotypes and Identifying Cultivars in Horticulture

Authors

M. Ravelonandro, P. Briard, R. Renaud, R. Scorza

Keywords

plum pox potyvirus, genetic engineering, protection, breeding.

Full text

https://doi.org/10.17660/ActaHortic.2001.546.79

Online Articles (90)

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2. GENETIC DISTANCE ESTIMATORS BASED ON MOLECULAR DATA FOR PLANT REGISTRATION AND PROTECTION: A REVIEW

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T.H. Han | H.J. van Eck | M.J. de Jeu | E. Jacobsen

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16. EVALUATION OF GENETIC DIVERSITY IN A GARDEN BEET GERMPLASM COLLECTION USING RAPD TECHNIQUE

R. Baranski | D. Grzebelus | L. Frese

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18. ASSESSMENT OF THE GENETIC RELATIONSHIPS AMONG CITRUS SPECIES AND VARIETIES BY ISOZYME AND RAPD MARKERS

L. Cabrita | P. Elisiário | J. Leitão | A. Guerreiro

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E. Coart | V. Lamote | J. De Riek | E. Van Bockstaele | M. De Loose

20. ESTIMATION OF HETEROZYGOSITY IN TWO BOTANIC ROSE SPECIES USING AFLP MARKERS

L. Crespel | D. Zhang | J. Meynet | S. Gudin

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22. CHARACTERIZATION OF MICROSATELLITE FLANKING SEQUENCES IN VITIS SPECIES

G. Di Gaspero | E. Peterlunger | R. Testolin | G. Cipriani

23. GENETIC RELATIONSHIPS BETWEEN APRICOT (Prunus armeniaca L.) AND RELATED SPECIES USING AFLP MARKERS

L.S. Hagen | P. Lambert | J.M. Audergon | B. Khadari

24. IDENTIFICATION OF APRICOT (Prunus armeniaca L.) GENOTYPES USING MICROSATELLITE AND RAPD MARKERS

J.I. Hormaza

25. CHARACTERIZATION OF ACER GRISEUM AND ITS PUTATIVE INTERSPECIFIC HYBRIDS

Y.H. Joung | M.S. Roh | S.E. Bentz

26. THE USE OF MICROSATELLITE MARKERS TO EXAMINE THE DIVERSITY OF THE GENETIC RESOURCES OF RICE (Oryza sativa) ADAPTED TO EUROPEAN CONDITIONS

C. Luce | J.L. Noyer | D. Tharreau | N. Ahmadi | H. Feyt

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F. Luro | D. Rist | P. Ollitrault

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29. GENETIC DIVERSITY OF A FERAL POPULATION OF OLIVE TREES SURROUNDING AN ABANDONED GROVE

G. Mekuria | G. Collins | M. Sedgley

30. GENETIC VARIATION IN WILD AND CULTIVATED MATERIAL OF BLACK CHOKEBERRY

H.A. Persson | N. Jeppsson | H. Nybom

31. S-HAPLOTYPE POLYMORPHISM IN BRASSICA OLERACEA

V. Ruffio-Chable | T. Gaude

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M. Tavaud | A. Zanetto | F. Santi | E. Dirlewanger

33. CHARACTERISATION OF GRAPEVINE ACCESSIONS AT GERMPLASM BANKS WITH RAPD AND MICROSATELLITE MARKERS

S. Ulanowsky | Y. Gogorcena | F. Martinez-de-Toda | J. Borrego | J. Ibanez

34. MOLECULAR MARKERS IN DETECTION OF DISTANT HYBRIDS IN LILIUM

K. Wiejacha | A. Marasek | I. Sabala | T. Orlikowska

35. POSSIBLE FUTURE ROLES FOR MOLECULAR TECHNIQUES IN THE IDENTIFICATION AND REGISTRATION OF NEW PLANT CULTIVARS

M.S. Camlin

36. COMPARISON OF FRUIT AND LEAF DNA EXTRACTS FOR AFLP AND SSR ANALYSIS IN PEACH (Prunus persica (L.) Batsch)

M.J. Aranzana | M.C. de Vicente | P. Arús

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G. Arnau | J. Lallemand | M. Bourgoin

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39. OLIVE CULTIVAR IDENTIFICATION USING NUCLEAR RAPDS AND MITOCHONDRIAL RFLPS

G. Besnard | P. Baradat | A. Bervillé

40. DNA FINGERPRINTS FOR PELARGONIUM CULTIVAR IDENTIFICATION

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F. Regner | A. Stadlbauer | C. Eisenheld

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D. Bellin | R. Velasco | M.S. Grando

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D. Zhang | C. Besse | M.Q. Cao | M.H. Gandelin

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M.J. Aranzana | P. Arús | J. Carbó | G.J. King

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G. Corbett | D. Lee | P. Donini | R.J. Cooke

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L. De Benedetti | A. Mercuri | S. Bruna | G. Burchi | T. Schiva

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I. Filippetti | O. Silvestroni | M.R. Thomas | C. Intrieri

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L.G. Fraser | C.F. Harvey | G.P. Gill

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M.L. Grant | D.M. Miller | A. Culham

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D. Grzebelus | R. Baranski | B. Jagosz | B. Michalik | P.W. Simon

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Y.H. Joung | M.S. Roh | T.I. Kim | J.S. Song

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K. Kenis | E. Pauwels | N. Van Houtvinck | J. Keulemans

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B. Khadari | C. Breton | G. Besnard | J.P. Roger | A. Bervillé

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B. Khadari | A. Bervillé

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S. Lanteri | I. Di Leo | E. Portis | L. Quagliotti

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G. Mennella | V. Onofaro Sanajà | A. D'Alessandro

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I. Métais | C. Aubry | B. Hamon | R. Jalouzot | D. Peltier

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A. Mohanty | J.P. Martín | I. Aguinagalde

64. POTATO CULTIVARS IDENTIFICATION USING MOLECULAR MARKERS

M. Moisan-Thiéry | Y. Le Hingrat | M.C. Kerlan

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C. Pankhurst | J. Compton | S. Thornton-Wood | J. Cubey | A. Culham

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G.R. Pratta | R. Zorzoli | L.A. Picardi | E.M. Valle | N. Carrillo

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R. Quarta | M.T. Dettori | I. Verde | U. Marchesi | M.A. Palombi

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C.M. Reis | A.M. Frederico

69. MOLECULAR MARKERS AND GENETIC BASE BROADENING IN LYCOPERSICON SPP.

G. Saavedra | W. Spoor | L. Harrier

70. DISTINCTION BETWEEN CLOSELYRELATED APPLE CULTIVARS OF THE BELLE-FLEUR FAMILY USING RFLP AND AFLP MARKERS

M. Tignon | M. Lateur | R. Kettmann | B. Watillon

71. IDENTIFICATION OF COPIA-LIKE RETROTRANSPOSABLE ELEMENT BY APPLE

M. Tignon | B. Watillon | R. Kettmann

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N. Van Stallen | V. Noten | M. Demeulemeester | M.P. De Proft

73. ROUTINE EXTRACTION OF DNA FROM GRAPEVINE (Vitis ssp.) CANES AND ROOTS FOR VARIETY IDENTIFICATION BY RAPD-PCR

T. Wolf | C. Ortlieb | K. Eimert | R. Ries

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D.P. Zhang | Z. Huaman | F. Rodriguez | G. Rossel | M. Ghislain

75. IDENTIFICATION OF CHINESE CABBAGE (Brassica campestris L. ssp. Pekinensis) CULTIVARS WITH ISOZYME, RAPD AND AFLP MARKERS

X. Zheng | S. Song | H. Liu

76. IDENTIFICATION OF GENOTYPES USING AFLP MARKERS IN APPLE ROOTSTOCKS

J. Zhu | A.Q. Zhou | H.Y. Dai | G.C. Li

77. MARKER-ASSISTED SELECTION FOR THE TRANSFER OF QTLS CONTROLLING FRUIT QUALITY TRAITS INTO TOMATO ELITE LINES

M. Causse | L. Lecomte | N. Baffert | P. Duffé | F. Hospital

78. IDENTIFICATION OF A RAPD MARKER LINKED TO TOLERANCE TO PEACOCK DISEASE (Spilocea oleaginea) IN OLIVES (Olea europaea)

G. Mekuria | G. Collins | S. Lavee | M. Sedgley

79. TRANSGENE-BASED RESISTANCE TO PLUM POX VIRUS (SHARKA DISEASE) IS TRANSFERRED THROUGH INTERSPECIFIC HYBRIDIZATION IN PRUNUS

M. Ravelonandro | P. Briard | R. Renaud | R. Scorza

80. MOLECULAR IDENTIFICATION OF S-GENOTYPES OF ALMOND (Prunus dulcis)

Ma | M.M. Oliveira

81. MARKER ASSISTED SELECTION FOR SCAB RESISTANT APPLE

L. Giongo | A. Bergamini | R. Rigatti | M. Komjanc

82. AFLP MARKERS FOR SEX DETERMINATION IN AN ILEX SPECIES

A. Golan-Goldhirsch | R. Jones | L.J. Rowland

83. DEVELOPMENT OF DNA MARKERS LINKED TO BACTERIAL LEAF SPOT RESISTANCE OF CHILLI

K.T. Kim | H.S. Choi | H.J. Kim | D.H. Pae | J.Y. Yoon

84. DETECTION OF BEAN SEED-BORNE PATHOGENS BY PCR

F. Molouba | C. Guimier | C. Berthier

85. USE OF DIFFERENT MOLECULAR MARKERS, RAPDS AND SSRS, TO INVESTIGATE CLONAL STABILITY IN MICROPROPAGATED KIWIFRUIT (Actinidia deliciosa A. Chev.)

M.A. Palombi | C. Damiano

86. IDENTIFICATION OF MOLECULAR MARKERS LINKED TO POWDERY MILDEW (Erysiphe pisi Syd.) RESISTANCE MUTATED GENES IN PISUM SATIVUM L.

G. Pereira | M.T. Sousa | J.M. Leitão

87. AFLP MARKER DEVELOPMENT IN ROSE FOR GENETIC MAPPING: COMPARISON OF THREE RESTRICTION ENZYME PAIRS

S. Rajapakse | L. Zhang | R.E. Ballard | D.H. Byrne

88. CHALLENGES IN DEVELOPING MOLECULAR MARKERS FOR ALMOND (Prunus amygdalus) AND GRAPE (Vitis species)

89. GENETIC ANALYSIS OF A POLISH APPLE SELECTION U 211 AS A NEW SOURCE OF HIGH RESISTANCE TO APPLE POWDERY MILDEW

M. Stankiewicz | E. Pitera | S.W. Gawronski

90. INTROGRESSION OF A PARTIAL RESISTANCE TO PHYTOPHTHORA CAPSICI LEON. INTO A PEPPER ELITE LINE BY MARKER ASSISTED BACKCROSSES