Articles
IDENTIFICATION OF A RAPD MARKER LINKED TO TOLERANCE TO PEACOCK DISEASE (Spilocea oleaginea) IN OLIVES (Olea europaea)
Article number
546_78
Pages
565 – 567
Language
Abstract
Olive leaf spot, peacock spot, and bird’s eye spot are names for the wide-spread disease of olives caused by the fungus Spilocea oleaginea. The symptoms of the disease are dark green or black circular spots, that develop mainly on the leaf blades.
A complete loss of yield of some sensitive cultivars can occur in certain climatic regions.
In the present study, progeny from a population segregating for resistance to peacock disease were obtained from a breeding program in Israel.
The DNA from a sample of this population was examined for a resistance marker using bulked segregant analysis (BSA) in conjunction with the random amplified polymorphic DNA (RAPD) technique.
Two DNA bulks were constructed, each derived from 15 progeny showing either tolerance or susceptibility for the disease.
About 100 random sequence Operon primers were used to screen for polymorphisms between the two bulks, and those primers that produced polymorphic PCR products between the two bulks were repeatedly tested for consistency.
Where a consistent polymorphism was produced, all parents and progeny were tested individually for the presence of the polymorphism.
Of the 100 primers screened, two showed polymorphic bands between the two bulks.
One of those produced a marker that was found in about 80% of both parents and progeny showing tolerance for peacock spot disease.
A complete loss of yield of some sensitive cultivars can occur in certain climatic regions.
In the present study, progeny from a population segregating for resistance to peacock disease were obtained from a breeding program in Israel.
The DNA from a sample of this population was examined for a resistance marker using bulked segregant analysis (BSA) in conjunction with the random amplified polymorphic DNA (RAPD) technique.
Two DNA bulks were constructed, each derived from 15 progeny showing either tolerance or susceptibility for the disease.
About 100 random sequence Operon primers were used to screen for polymorphisms between the two bulks, and those primers that produced polymorphic PCR products between the two bulks were repeatedly tested for consistency.
Where a consistent polymorphism was produced, all parents and progeny were tested individually for the presence of the polymorphism.
Of the 100 primers screened, two showed polymorphic bands between the two bulks.
One of those produced a marker that was found in about 80% of both parents and progeny showing tolerance for peacock spot disease.
Authors
G. Mekuria, G. Collins, S. Lavee, M. Sedgley
Keywords
Olea, olive, Spilocea oleaginea, peacock spot, bulked segregant analysis, DNA marker
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