Articles
STABLE TRANSFER OF THE PLUM POX VIRUS (PPV) CAPSID TRANSGENE TO SEEDLINGS OF TWO FRENCH CULTIVARS ‘PRUNIER D’ENTE 303’ AND ‘QUETSCHE 2906’, AND PRELIMINARY RESULTS OF PPV CHALLENGE ASSAYS
Article number
577_12
Pages
91 – 96
Language
English
Abstract
Transgenic plum clone C-5 has been shown to be highly resistant to PPV. The C-5 clone displays post-transcriptional gene silencing (PTGS), a plant defense commonly described in herbaceous plant models.
The PPV-CP transgene in C-5 was transferred through hybridization to seedlings of two commonly grown French plum cultivars.
The male (pollen) parent was C-5 and Prunier dEnte 303 and Quetsche 2906 were females.
Histochemical GUS assays and molecular analyses revealed that almost 45% of the hybrids were transgenic.
Evaluation of PPV infection in selected seedling clones showed that these transgenic hybrids displayed a resistant phenotype similar to that of the parental C-5 clone.
C-5 plum represents useful transgenic germplasm for developing, through classical breeding, new plum varieties highly resistant to PPV.
The PPV-CP transgene in C-5 was transferred through hybridization to seedlings of two commonly grown French plum cultivars.
The male (pollen) parent was C-5 and Prunier dEnte 303 and Quetsche 2906 were females.
Histochemical GUS assays and molecular analyses revealed that almost 45% of the hybrids were transgenic.
Evaluation of PPV infection in selected seedling clones showed that these transgenic hybrids displayed a resistant phenotype similar to that of the parental C-5 clone.
C-5 plum represents useful transgenic germplasm for developing, through classical breeding, new plum varieties highly resistant to PPV.
Authors
M. Ravelonandro, P. Briard, M. Monsion, R. Scorza
Keywords
breeding, inheritance, potyvirus resistance, Prunus domestica, sharka, transgenics
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