Articles
THE EFFECT OF COMMERCIAL BRASSINOSTEROID ON GROWTH AND STEROIDAL GLYCOALKALOID CONTENT OF CULTIVATED POTATO (SOLANUM TUBEROSUM) AND
Article number
619_35
Pages
309 – 313
Language
English
Abstract
Leaves of cultivated potato (Solanum tuberosum) contain the steroidal glycoalkaloids (SGAs) solanine and chaconine, whereas leaves of certain accessions of wild S. chacoense contain solanine, chaconine, leptinines and leptines.
Leptines are the primary source of natural resistance in S. chacoense to herbivory by larvae and adult Colorado potato beetles (Leptinotarsa decemlineata Say). The aglycons of solanine, chaconine, leptinines and leptines are solanidine (SD), leptinidine (LD) and acetylleptinidine (ALD), respectively.
On a dry weight basis, leaves of field- and greenhouse-grown plants contain relatively higher (four to ten times) levels of the SGAs (measured as SD, LD and ALD) than leaves of plants grown in vitro on MS medium lacking hormones.
In addition, leaves from field-grown S. chacoense plants contain more ALD than SD, while leaves from in vitro-grown plants contain more SD than ALD. Previous experiments have shown that a variety of plant growth regulators have small but significant effects on the SGA quantity and/or ratio of in vitro-grown S. tuberosum or S. chacoense. In new experiments intended to understand the regulation of SGA levels in S. tubersum and S. chacoense, plantlets were grown in vitro for 30 days on MS medium containing 0 to 1000 µl of a commercial brassinosteroid preparation (Tianfengsu; Jiangmen Pesticide Factory, P.R. China). Highest brassinosteroid levels (100 or 1000 µl) significantly inhibited growth of S. tuberosum and S. chacoense plantlets compared to controls lacking brassinosteroid.
Lower brassinosteroid levels (1 µl, 10 µl) significantly enhanced plantlet growth.
Brassinosteroid, regardless of amount added, had no effect on foliar SGA levels of S. tuberosum or S. chacoense.
Leptines are the primary source of natural resistance in S. chacoense to herbivory by larvae and adult Colorado potato beetles (Leptinotarsa decemlineata Say). The aglycons of solanine, chaconine, leptinines and leptines are solanidine (SD), leptinidine (LD) and acetylleptinidine (ALD), respectively.
On a dry weight basis, leaves of field- and greenhouse-grown plants contain relatively higher (four to ten times) levels of the SGAs (measured as SD, LD and ALD) than leaves of plants grown in vitro on MS medium lacking hormones.
In addition, leaves from field-grown S. chacoense plants contain more ALD than SD, while leaves from in vitro-grown plants contain more SD than ALD. Previous experiments have shown that a variety of plant growth regulators have small but significant effects on the SGA quantity and/or ratio of in vitro-grown S. tuberosum or S. chacoense. In new experiments intended to understand the regulation of SGA levels in S. tubersum and S. chacoense, plantlets were grown in vitro for 30 days on MS medium containing 0 to 1000 µl of a commercial brassinosteroid preparation (Tianfengsu; Jiangmen Pesticide Factory, P.R. China). Highest brassinosteroid levels (100 or 1000 µl) significantly inhibited growth of S. tuberosum and S. chacoense plantlets compared to controls lacking brassinosteroid.
Lower brassinosteroid levels (1 µl, 10 µl) significantly enhanced plantlet growth.
Brassinosteroid, regardless of amount added, had no effect on foliar SGA levels of S. tuberosum or S. chacoense.
Authors
A.R. Miller, J.C. Jang, N.J. Flickinger
Keywords
Colorado potato beetle, leptines, solanine, chaconine, leptinines, tissue culture
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