Articles
CALLUS INDUCTION AND PLANT REGENERATION IN VITRO IN POTATO
Article number
619_36
Pages
315 – 322
Language
English
Abstract
This study was conducted to determine the effects of growth regulators (2,4-D and kinetin), cultivar, explant and light on callus induction in potato.
In addition, the effects of cultivar and explant were evaluated on callus organogenesis.
At first callus induction on internode explants was tested using MS medium supplemented with combination of 1, 2 or 3 mg L-1 2,4-D and 0.00, 0.01 or 0.10 mg L-1 kinetin.
Analysis of variance revealed a significant effect of 2, 4-D and kinetin concentration, and their interaction, on frequency of callus induction and number of roots on callus.
Also the effects of kinetin concentration and kinetinx2,4-D interaction were significant on the initiation time of callus induction and volume of callus.
However, the effect of 2,4-D concentration alone on these variables was not significant.
The effects of cultivar and explant on callus induction in leaf and internode explants of six potato cultivars (Agria, Cosmos, Sante, Concord, Ajax and Diamant) were studied.
Leaf and internode explants were planted on MS medium, supplemented with 5 mg L-1 2,4-D and 0.25 mg L-1 kinetin.
Analysis of variance revealed significant effect of cultivar and cultivarxexplant interaction on callus volume, while the effect of explant was not significant.
The effect of cultivar, explant and their interaction on frequency of callus induction was not significant, while the effects of these factors on the initiation time of callus induction was significant.
Finally, the effect of light on callus induction in leaf and internode explants was investigated.
Callus was mostly induced in leaf explants under dark conditions, but was induced in internode explants under both dark and light conditions.
Also in the regeneration stage, the effects of cultivar and explant on callus organogenesis were significant.
In addition, the effects of cultivar and explant were evaluated on callus organogenesis.
At first callus induction on internode explants was tested using MS medium supplemented with combination of 1, 2 or 3 mg L-1 2,4-D and 0.00, 0.01 or 0.10 mg L-1 kinetin.
Analysis of variance revealed a significant effect of 2, 4-D and kinetin concentration, and their interaction, on frequency of callus induction and number of roots on callus.
Also the effects of kinetin concentration and kinetinx2,4-D interaction were significant on the initiation time of callus induction and volume of callus.
However, the effect of 2,4-D concentration alone on these variables was not significant.
The effects of cultivar and explant on callus induction in leaf and internode explants of six potato cultivars (Agria, Cosmos, Sante, Concord, Ajax and Diamant) were studied.
Leaf and internode explants were planted on MS medium, supplemented with 5 mg L-1 2,4-D and 0.25 mg L-1 kinetin.
Analysis of variance revealed significant effect of cultivar and cultivarxexplant interaction on callus volume, while the effect of explant was not significant.
The effect of cultivar, explant and their interaction on frequency of callus induction was not significant, while the effects of these factors on the initiation time of callus induction was significant.
Finally, the effect of light on callus induction in leaf and internode explants was investigated.
Callus was mostly induced in leaf explants under dark conditions, but was induced in internode explants under both dark and light conditions.
Also in the regeneration stage, the effects of cultivar and explant on callus organogenesis were significant.
Authors
M. Omidi, A. Shahpiri
Keywords
Growth regulators, Cultivar, Explant, Light, Callus organogenesis
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